中西医结合肝病杂志2026，Vol.36Issue(2)：149-153,5.DOI:10.3969/j.issn.1005-0264.2026.002.004
miR-363-3p与FZD10在肝细胞癌中的表达及临床病理特征与预后的关系
The relationship between the expression of miR-363-3p and FZD10 in hepatocellular carcinoma tissues and the clinical pathological characteristics and prognosis
摘要
Abstract
Objective:To investigate the expression of microRNA-363-3p(miR-363-3p)and frizzled 10(FZD10)in liver cancer tissue and their relationship with clinical pathological features and prognosis.Methods:This study selected 86 liver cancer patients who were treated in our hospital from January 2018 to January 2021,and the excised cancer tissue and adjacent tissues during surgery were used as experimental materials.Real-time fluorescence quantitative PCR was applied to detect miR-363-3p and FZD10 mRNA levels.Immunohistochemical staining(IHC)was applied to detect FZD10 protein level.Pearson correlation was applied to analyze the correlation between miR-363-3p and FZD10 mRNA.StarBase website was applied to predict the binding sites of miR-363-3p and FZD10.Kaplan-Meier was applied for survival analysis.COX regression was applied to analyze the influencing factors of poor prognosis.Results:miR-363-3p was highly expressed in adjacent cancer tissues,while FZD10 mRNA and FZD10 protein were highly expressed in cancer tissues(P＜0.05).The expression of miR-363-3p and FZD10 mRNA was negatively correlated(r=-0.455,P=0.000),and StarBase prediction showed a binding site between miR-363-3p and FZD10 mRNA.The expression of miR-363-3p and FZD10 in cancer tissue was correlated with microvascular invasion,intrahepatic metastasis,cirrhosis,and lymph node status in patients(P＜0.05).The cumulative survival rate of patients with high expression of miR-363-3p was 78.57％(33/42),which was higher than 45.45％(20/44)of patients with low expression of miR-363-3p.The cumulative survival rate of patients with high expression of FZD10 was 48.83％(22/48),which was lower than 81.58％(31/38)of patients with low expression of FZD10(P＜0.05).miR-363-3p,FZD10,intrahepatic metastasis,cirrhosis,and lymph node status were independent influencing factors for poor prognosis in patients(P＜0.05).Conclusion:miR-363-3p is poorly expressed in cancer tissue,while FZD10 mRNA and protein are highly expressed in cancer tissue.Both are associated with clinical pathological features such as microvascular invasion,intrahepatic metastasis,cirrhosis,and lymph node status.Patients with low expression of miR-363-3p and high expression of FZD10 have lower survival rates.关键词
肝细胞癌/miRNA-363-3p/卷曲类受体10/临床病理特征/预后Key words
hepatocellular carcinoma/micro RNA-363-3p/frizzled 10/clinical pathological features/prognosis分类
医药卫生引用本文复制引用
贾会文,刘赟,徐赟,杨启,杨科..miR-363-3p与FZD10在肝细胞癌中的表达及临床病理特征与预后的关系[J].中西医结合肝病杂志,2026,36(2):149-153,5.基金项目
2024年度河南医学科学项目(No.2024-YJ-169) （No.2024-YJ-169）
