种子2026，Vol.45Issue(2)：41-48,8.DOI:10.16590/j.cnki.1001-4705.2026.02.041
高粱RIL群体高密度遗传图谱的构建及株高QTL定位
Construction of a High-Density Genetic Map and QTL Mapping for Plant Height in a Sorghum bicolor RIL Population
摘要
Abstract
The height of Sorghum bicolor plants is a key agronomic trait that affects its resistance to lodging and the efficiency of mechanized harvesting.To understand its genetic basis,a 235 recombinant inbred line(RIL)population constructed by the dwarf variety Henong16 and the tall wine Sorghum bicolor Hongyinzi was used as the material.Using whole-genome resequencing technology,a high-density genetic map contai-ning 2 822 Bin markers was constructed,with an average map distance of 0.87 cM.Through phenotypic iden-tification at 4 points over 2 years,18 height-related QTLs were detected on chromosomes 1,3,4,5,6,7,and 9.Among them,6 QTLs were stable in at least 2 environments,with qPH9.1 having the highest contri-bution rate of 38.89％.Co-localization analysis indicated that qPH7.1 and qPH9.1 overlapped or were closely linked to the known major-effect genes Dw3 and Dw1,respectively.Additionally,6 new candidate genes were predicted within the remaining stable QTL intervals,such as SbiHYZ.01G060500 homologous to rice casein kinase CKI and SbiHYZ.03G385400 homologous to bZIP transcription factor OsABF1.These genes were mainly involved in hormone signaling pathways such as gibberellin and the regulation of growth period.关键词
高粱/株高/SNP标记/QTL定位/遗传图谱Key words
Sorghum bicolor/plant height/SNP marker/quantitative trait locus(QTL)/genetic mapping分类
农业科技引用本文复制引用
杨沛东,程斌,高旭,徐建霞,曹宁,李文贞,丁延庆,张立异..高粱RIL群体高密度遗传图谱的构建及株高QTL定位[J].种子,2026,45(2):41-48,8.基金项目
贵州省基础研究计划(自然科学)项目(黔科合基础[2024]青年077) （自然科学）
特色杂粮分子育种贵州省重点实验室项目(黔科合平台ZSYS[2025]026) （黔科合平台ZSYS[2025]026）
国家自然科学基金项目(32460493、32572425) （32460493、32572425）
贵州省基础研究计划(自然科学)面上项目(黔科合基础zk[2025]面上299) （自然科学）
