种子2026，Vol.45Issue(2)：49-55,7.DOI:10.16590/j.cnki.1001-4705.2026.02.049
漯系列小麦品种(系)品质及抗逆基因的KASP检测与分析
KASP Detection and Analysis of Quality and Stress Resistance Genes in Luo Series Wheat Varieties(Lines)
摘要
Abstract
Using 4 wheat(Triticum aestivum L.)varieties(lines)as materials,the KASP marker tech-nology combined with conventional quality testing was employed to analyze the genetic composition of their quality traits and disease-resistance and stress-tolerance genes.The quality analysis indicated that Luomai83 met the standard of medium-strong gluten wheat,while the other materials were of medium-gluten type.The KASP test revealed that in terms of quality genes,all 4 varieties contained the hard type gene Pinb-D1b and the high-quality subunit gene Ax1/Ax2*.Luomai83 combined Dx5+Dy10 with the Ax1/Ax2*high-quality subunit gene,explaining the molecular mechanism of its strong gluten phenotype.In terms of flour color,all 4 varieties carried the gene Lox-B1a with the enhancing whitening effect of high-fat oxidation enzyme activity.In terms of disease-resistance and stress-tolerance genes,the anti-spike germination gene TaSdr-B1a was distributed at a frequency of 100％in the tested materials,while the anti-leaf rust gene Lr16,the excellent allele COMT-3Ba with high lignin content,and the anti-spike germination gene Rio Blanco type were utilized to a higher extent in the 4 varieties(lines),and the anti-barley yellow rust gene Yr78,the drought-resistant gene Westonia type and TaDreb-B1a were utilized to a lower extent;no anti-leaf rust genes Lr46 and Lr48,anti-powdery mildew genes Pm21,and anti-mold blight genes Fhb1 were detected.Luomai83 contained 2 loci of high-quality subunit genes(5+10)+Ax1/Ax2*,and the quality test results were medium-strong gluten,and it also combined 4 disease-resistance and stress-tolerance genes,which could be used as excellent parental materials for wheat breeding.关键词
小麦/品质基因/抗病基因/抗逆基因/KASP检测Key words
Triticum aestivum/quality genes/disease resistant genes/stress resistance genes/KASP detection分类
农业科技引用本文复制引用
王君,黄杰,曹燕燕,葛昌斌,张振永,廖平安..漯系列小麦品种(系)品质及抗逆基因的KASP检测与分析[J].种子,2026,45(2):49-55,7.基金项目
国家现代农业产业技术体系建设专项(CARS-03) （CARS-03）
河南省农业良种联合攻关项目(2022010101) （2022010101）
河南省中央引导地方科技发展资金(Z20231811053) （Z20231811053）
国家重点研发计划(2024YFD1400404-04) （2024YFD1400404-04）
