中药药理与临床2026，Vol.42Issue(2)：68-76,9.
基于肠道菌群和代谢组学探讨复方蜘蛛香凝胶贴膏治疗消化不良的作用机制
Therapeutic Mechanism of Compound Zhizhuxiang Gel Plaster on Dyspepsia Based on Gut Microbiota and Metabonomics
摘要
Abstract
Objective:To explore the mechanism of Compound Zhizhuxiang(蜘蛛香)Gel Plaster in the treatment of dyspepsia based on gut microbiota and metabolomics techniques.Methods:A high-calorie-diet-induced dyspepsia(HC-DID)model was established in young mice.The therapeutic effect of Compound Zhizhuxiang Gel Plaster on dys-pepsia was evaluated based on changes in body weight and food intake,gastric emptying rate,small intestinal propulsion rate,and pathological changes in the gastric antrum and duodenum.ELISA was used to detect the serum levels of D-xy-lose,cholecystokinin(CCK),vasoactive intestinal peptide(VIP),5-hydroxytryptamine(5-HT),and motilin(MTL).Changes in gut microbiota and metabolites in intestinal contents were analyzed by 16S rDNA high-throughput sequencing and ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS).Results:Compared with the normal control group,the model control group showed significantly reduced food intake and body weight,as well as suppressed gastric emptying rate and small intestinal propulsion rate(P<0.05).Compared with the model control group,Compound Zhizhuxiang Gel Plaster significantly increased food intake and body weight,enhanced gastric emptying and small intestinal propulsion rates,and promoted gastrointestinal motility in dyspeptic mice(P<0.05).Moreover,Compound Zhizhuxiang Gel Plaster increased the diversity of gut microbiota in dyspeptic mice.A total of 30 differential metabolites were identified in the intestinal contents of young mice,and Compound Zhizhuxiang Gel Plaster could restore metabolic disturbances.Five metabolic pathways,including arginine and proline metabolism,were i-dentified as the most significantly altered after intervention.Conclusion:Compound Zhizhuxiang Gel Plaster can improve food intake and promote gastrointestinal motility in dyspeptic young mice.Its mechanism may be closely related to the regulation of gut microbiota composition and arginine and proline metabolism.关键词
复方蜘蛛香凝胶贴膏/消化不良/D-木糖/胆囊收缩素/血管活性肠肽/5-羟色胺(5-HT)/胃动素/肠道菌群/代谢组学Key words
Compound Zhizhuxiang Gel Plaster/Dyspepsia/D-xylose/cholecystokinin(CCK)/vasoactive intestinal pep-tide(VIP)/5-hydroxytryptamine(5-HT)/motilin(MTL)/Gut microbiota/Metabolomics引用本文复制引用
梅佳华,查学志,孙杏倩,刘云宽,蔡俊飞,林维川,马云淑..基于肠道菌群和代谢组学探讨复方蜘蛛香凝胶贴膏治疗消化不良的作用机制[J].中药药理与临床,2026,42(2):68-76,9.基金项目
国家自然科学基金资助项目(编号:82174065) （编号:82174065）
云南省科技厅社会发展专项-重点研发计划项目(编号:202303AC100025) （编号:202303AC100025）
云南省教育厅科学研究基金项目(编号:2024Y379) （编号:2024Y379）
云南省中西医结合慢病防治重点实验室开放基金资助项目(编号:YPKLG2024-016) （编号:YPKLG2024-016）
云南省傣医药与彝医药重点实验室资助项目(编号:2024SS24074) （编号:2024SS24074）
国家中医药管理局"十二五"重点学科—傣药学. （）
