中药药理与临床2026，Vol.42Issue(2)：84-89,6.
女贞子多糖对H22荷瘤小鼠的抗肿瘤作用及机制研究
Antitumor Effect and Mechanism of Ligustrum lucidum Polysaccharide in H22 Tumor-Bearing Mice
摘要
Abstract
Objective:To investigate the antitumor effect and mechanism of Ligustrum lucidum polysaccharide in H22 tumor-bearing mice.Methods:The mouse model of H22 hepatocellular carcinoma was established.After 14 days of continuous administration,mice were dissected and tumor weights were measured to calculate the tumor inhibition rate.Serum levels of tumor necrosis factor(TNF)-α,interleukin(IL)-6,and interferon(IFN)-γ were determined by ELISA.Annexin-V-PE/7AAD dual-staining flow cytometry was employed to assess tumor cell apoptosis and expression of the au-tophagy and apoptosis marker microtubule-associated protein 1 light chain 3 A/B(LC3A/B).Western blot was em-ployed to evaluate the expression levels of the anti-apoptotic protein B-cell lymphoma 2(Bcl2),the pro-apoptotic protein Bcl2-associated X protein(Bax),LC3Ⅱ/Ⅰ,beclin1,protein kinase B(AKT),and mammalian target of rapamycin(mTOR).Results:L.lucidum polysaccharide exhibited significant antitumor activity,achieving a tumor inhibition rate of 50.83%.Compared with the model group,the L.lucidum polysaccharide(20 mg/kg)group showed decline serum levels of TNF-α,IL-6,and IFN-γ(P<0.01 or P<0.05),increased tumor cell apoptosis,up-regulated Bax expression,and down-regulated Bcl2 expression(P<0.05).In addition,this group showed increases in the percentage of LC3A/B and LC3Ⅱ/Ⅰ,and the expression levels of beclin1,reduces in the expression levels of AKT and mTOR(P<0.05,P<0.01).Conclusion:L.lucidum polysaccharide can inhibit the tumor growth and promote the tumor cell apoptosis and auto-phagy in H22 tumor-bearing mice by inhibiting the AKT-mTOR signaling pathway.关键词
女贞子多糖/H22荷瘤小鼠/凋亡/自噬/蛋白激酶B/哺乳动物雷帕霉素靶蛋白信号通路Key words
Ligustrum lucidum polysaccharide/H22 tumor-bearing mice/Apoptosis/Autophagy/Protein kinase B(AKT)-mammalian target of rapamycin(mTOR)pathway引用本文复制引用
史晶晶,时博..女贞子多糖对H22荷瘤小鼠的抗肿瘤作用及机制研究[J].中药药理与临床,2026,42(2):84-89,6.基金项目
河南省高等学校青年骨干教师培养计划项目(编号:2020GGJS108) （编号:2020GGJS108）
河南省科技攻关项目(编号:242102310547). （编号:242102310547）
