安徽大学学报（自然科学版）2026，Vol.50Issue(2)：66-79,14.DOI:10.3969/j.issn.1000-2162.2026.02.009
基于转录组和SNP标记研究草珊瑚群体遗传分化
Genetic differentiation of Sarcandra glabra populations based on transcriptome and SNP analyses
摘要
Abstract
The extensive collection of Sarcandra glabra by the pharmaceutical industry necessitates a re-evaluation of the wild germplasm resources for the traditional medicinal herb.However,there have been no studies on large-scale S.glabra populations based on single nucleotide polymorphisms(SNPs)to date.In this study,the transcriptomes of 16 collected individuals were sequenced,and 7 605 high-quality SNPs were developed.Based on the analyses of SNPs with three different methods,the samples were divided into two distinct groups,namely the temperate and tropical groups.Between the two groups,2 326 genes demonstrated significant variations in allele frequency,2 460 genes were differentially expressed,and this intersection of the two gene sets contains 19 genes.A subset of the SNPs were validated by using MassARRAY with a call rate of 61.9％,which can be utilized for further exploration and conservation management of S.glabra.A significant correlation between genetic distance and geographical distance among different samples suggests that the genetic differentiation may primarily be attributed to geographical isolation.关键词
草珊瑚/转录组/单核苷酸多态性/群体遗传/进化Key words
Sarcandra glabra/transcriptome/single nucleotide polymorphism/population genetics/evolution分类
生物科学引用本文复制引用
任超,张务龙,何柳燕,张璐..基于转录组和SNP标记研究草珊瑚群体遗传分化[J].安徽大学学报（自然科学版）,2026,50(2):66-79,14.基金项目
国家自然科学基金资助项目(31700286) （31700286）
