安徽大学学报（自然科学版）2026，Vol.50Issue(2)：89-100,12.DOI:10.3969/j.issn.1000-2162.2026.02.011
复方菌草灵芝保健茶超声辅助水提工艺优化及其免疫调节功能评价
Optimization of ultrasonic-assisted water extraction process of compound Juncao Ganoderma lucidum health tea and its immunomodulation function evaluation
摘要
Abstract
Single factor and orthogonal experiments were used to optimize the water extraction process,with the extract rate and polysaccharide content as the indexes for comprehensive evaluation.The main components of the dry extract powder of FJLBC were analyzed by infrared spectroscopy.Four indicators including the immune organ index were used to investigate the regulatory effect of FJLBC on the immune function of mice.The results showed that the best ultrasonic-assisted water extraction process for FJLBC was that the ratio of raw material to distilled water was 1∶25(g·mL-1),immersion time was 4 h,extraction temperature was 80℃,extraction time was 2.5 h,and number of extractions were 2.Infrared spectroscopy analysis showed that the dry extract powder of FJLBC contained polysaccharides,tea polyphenols and flavonoids,and the contents of which were 182.0,135.8 and 17.4 mg·g-1,respectively.The low-dose group(4.065 g·kg-1·d-1)and middle-dose group(8.13 g·kg-1·d-1)FJLBC could significantly improve mouse spleen and thymus indexes.The low-dose,middle-dose and high-dose groups(16.26 g·kg-1·d-1)FJLBC could in different degrees increase the levels of cytokines IL-10,IL-2 and INF-γ in mice.The low-dose FJLBC could significantly increase the number of hemolytic plaques,increase the phagocytic rate and phagocytic index of chicken red blood cells by macrophages,and promote the proliferation and differentiation of lymphocytes in mouse spleen and thymus,and thus increase the number of antibody-forming cells,enhance the phagocytic function of macrophages,and increase the immunomodulation function of mice.The best formula of each portion of FJLBC health tea was screened as follows:38.4 g Juncao Ganoderma lucidum,12.6 g selenium-enriched tea and 1.26 g perilla.关键词
水提工艺/浸膏得率/正交试验/红外光谱/免疫调节Key words
water extraction process/extract rate/orthogonal test/infrared spectrum/immune regulation分类
轻工纺织引用本文复制引用
张桂清,杨宇森,梁志豪,王星晨,罗海凌,姚俊新,刘斌,林占熺,钟路生..复方菌草灵芝保健茶超声辅助水提工艺优化及其免疫调节功能评价[J].安徽大学学报（自然科学版）,2026,50(2):89-100,12.基金项目
福建省科技厅引导性项目(2022N0011) （2022N0011）
福建省科学技术厅高校产学合作项目(2015Y4001) （2015Y4001）
福建省科技厅科技特派员后补助项目(2022S2089) （2022S2089）
