安徽医科大学学报2026，Vol.61Issue(1)：9-15,7.DOI:10.19405/j.cnki.issn1000-1492.2026.01.003
油酸诱导的脂滴合成对骨肉瘤细胞增殖、迁移、侵袭和上皮-间质转化的影响
Effects of oleic acid-induced lipid droplet synthesis on the proliferation,migration,invasion,and epithelial-mesenchymal transition of osteosarcoma cells
摘要
Abstract
Objective To explore the effects of different concentrations of oleic acid on human osteosarcoma cell lines 143B and HOS,as well as the impacts of the optimal concentration of oleic acid on cellular lipid droplet syn-thesis and cell functions.Methods The 143B and HOS cells were treated with varying concentrations of oleic acid(0,25,50,100,and 200 µmol/L)for 48 hours.Following treatment,oil red O staining and BODIPY staining were performed to determine the optimal concentration.Subsequently,CCK-8 assays and colony formation experi-ments were conducted to assess the effect of this optimal concentration of oleic acid on the cell proliferation of both cell lines.Transwell migration assays were utilized to evaluate the influence of the optimal concentration on migra-tory capacity and Transwell invasion assays were utilized to evaluate the invasive ability.Additionally,Western blot analysis was employed to examine the expression levels of epithelial-mesenchymal transition(EMT)markers Epithelial cadherin(E-cadherin)and Neural cadherin(N-cadherin)in response to treatment with the optimal con-centration of oleic acid.Results Treatment with oleic acid did not induce significant cell death in either 143B or HOS cells;however,an increase in intracellular lipid droplets was observed alongside enhanced proliferation,mi-gration,invasion capabilities as well as EMT transformation potential(P<0.05).Conclusion Oleic acid induces lipid droplet synthesis in osteosarcoma cells which subsequently promotes their proliferation,migration and inva-sion abilities along with EMT transformation.关键词
人骨肉瘤细胞株/脂滴合成/脂质代谢/油酸/增殖/迁移/侵袭Key words
human osteosarcoma cell line/lipid droplet synthesis/lipid metabolism/oleic acid/proliferation/migration/invasion分类
医药卫生引用本文复制引用
王梦婷,王云龙,梁梦霞,刘俊,卞尔保..油酸诱导的脂滴合成对骨肉瘤细胞增殖、迁移、侵袭和上皮-间质转化的影响[J].安徽医科大学学报,2026,61(1):9-15,7.基金项目
国家自然科学基金项目(编号:81972348) National Natural Science Foundation of China(No.81972348) （编号:81972348）
