安徽医科大学学报2026，Vol.61Issue(1)：23-29,7.DOI:10.19405/j.cnki.issn1000-1492.2026.01.005
不同浓度低氧影响少突胶质前体细胞迁移能力
Effects of hypoxia at different concentrations on the migration capacity of oligodendrocyte progenitor cells
摘要
Abstract
Objective To explore the effects of hypoxia on the migration ability of human oligodendrocyte precur-sor cells(hOPCs)and its regulatory mechanisms.Methods Based on the variations in oxygen concentration within the culture system,three experimental groups were set up:the 21%O₂ group(normoxic control group),the 5%O₂ group,and the 2%O₂ group.The migration ability of hOPCs under normoxia(21%O₂),5%O₂,and 2%O₂conditions was detected through the Transwell migration assay.RT-qPCR,transcriptome sequencing,and flow cy-tometry were used to detect the expression changes of genes and proteins such as hypoxia-inducible factor 1 alpha(HIF-1α)and chemokine(C-X-C Motif)receptor 4(CXCR4).Bioinformatics analysis was combined to analyze the KEGG pathways related to migration,so as to explore the effects of different oxygen concentrations on the migra-tion ability of hOPCs and their possible mechanisms.Results Hypoxia treatments at concentrations of 5%O₂ and 2%O₂ could both promote the in vitro migration of hOPCs,and the promoting effect of migration was more signifi-cant at the 2%O₂ concentration(P<0.001).After hypoxia treatment,the mRNA expression levels of HIF-1α,CXCR4,etc.in hOPCs significantly increased(P<0.001).Compared with the 5%O₂ concentration,the expres-sion of CXCR4 in cells was higher at the 2%O₂ concentration(P<0.000 1).Flow cytometry analysis detection showed that the expression of CXCR4 increased significantly after hypoxia treatment(P<0.01),and with the de-crease of oxygen concentration,its expression level further increased(P<0.000 1).Ordinary transcriptome se-quencing analysis indicated that hypoxia treatment could activate the PI3K-Akt signaling pathway and the Axon guidance pathway.Conclusion Hypoxia treatment can enhance the in vitro migration ability of hOPCs,and this effect is negatively correlated with the oxygen concentration.Its mechanism may be related to the up-regulation of the expression of genes such as HIF-1α and CXCR4,and the activation of the migration related signaling pathway including PI3K-Akt signaling pathway and axon guidance pathway.关键词
人少突胶质前体细胞/低氧/迁移/缺氧诱导因子-1α/趋化因子受体4/PI3K-Akt信号通路/轴突引导通路Key words
human oligodendrocyte precursor cells/low oxygen/migration/hypoxia-inducible factor 1 alpha/chemokine(C-X-C motif)receptor 4/PI3K-Akt signaling pathway/axon guidance pathway分类
生物科学引用本文复制引用
王倩,汪兆艳,栾佐,袁玉华..不同浓度低氧影响少突胶质前体细胞迁移能力[J].安徽医科大学学报,2026,61(1):23-29,7.基金项目
国家重点研发计划项目(编号:2017YFA0104203) National Key Research and Development Program of China(No.2017YFA0104203) （编号:2017YFA0104203）
