安徽医科大学学报2026，Vol.61Issue(1)：60-66,7.DOI:10.19405/j.cnki.issn1000-1492.2026.01.010
溶血型表皮葡萄球菌的微生物学特征研究
Microbiological characterization of Staphylococcus epidermidis with hemolytic phenotype
摘要
Abstract
Objective To explore the microbiological characteristics of Staphylococcus epidermidis with hemolytic phenotype(SEHP).Methods Hemolytic phenotype was detected using the three-point inoculation method,in-volving a total of 5 strains of SEHP and 5 strains of Staphylococcus epidermidis with non-hemolytic phenotype(SENHP).Bacterial species were identified using the Microflex LT MALDI-TOF mass spectrometer,and a phylo-genetic tree was constructed through 16S rRNA sequence alignment.Growth curves were monitored through the mi-crocultivation assay.Biofilm formation ability was assessed by microplate crystal violet staining.Red blood cell tox-icity was detected using the microplate method.Antimicrobial susceptibility testing of SEHP and SENHP against commonly used antibiotics was performed using a VITEK 2 GP639 test kit.Antagonistic effects of SEHP and SENHP against Staphylococcus aureus and Corynebacterium striatum were evaluated by the Oxford cup inhibition as-say.Results Compared with SENHP,SEHP exhibited a marked decrease in growth rate during the late logarith-mic phase,accompanied by significant hemolytic toxicity.Additionally,it showed lower resistance rates to levo-floxacin and moxifloxacin,and could antagonize Staphylococcus aureus and Corynebacterium striatum.Conclu-sion The microbiological characteristics of SEHP differ from those of SENHP in that SEHP demonstrates antago-nistic effects against S.aureus and C.striatum.关键词
表皮葡萄球菌/溶血表型/微生物学特征/耐药性/拮抗作用/生长曲线/人红细胞毒性Key words
Staphylococcus epidermidis/hemolytic phenotype/microbiological characteristics/antibiotic resis-tance/antagonistic effects/growth curve/toxicity to human erythrocytes分类
医药卫生引用本文复制引用
冷贵云,陈苇,王成浩,姚杰,陈传平,唐伟..溶血型表皮葡萄球菌的微生物学特征研究[J].安徽医科大学学报,2026,61(1):60-66,7.基金项目
国家自然科学基金项目(编号:82102460) （编号:82102460）
安徽医科大学研究生科研与实践创新项目(编号:YJS20240100) （编号:YJS20240100）
安徽省教育厅自然科学重点研究项目(编号:2022AH053046) National Natural Science Foundation of China(No.82102460) （编号:2022AH053046）
Graduate Student Scientific Research and Practical Innovation Project of Anhui Medical University(No.YJS20240100) （No.YJS20240100）
Natural Science Re-search Project of Anhui Educational Committee(No.2022AH053046) （No.2022AH053046）
