安徽医科大学学报2026，Vol.61Issue(2)：277-284,8.DOI:10.19405/j.cnki.issn1000-1492.2026.02.013
RUNX3对肝星状细胞活化增殖和迁移能力的影响
Effect of RUNX3 on the activation,proliferation,and migration capabilities of hepatic stellate cells
摘要
Abstract
Objective To investigate the effects of targeted silencing of Runt-related Transcription Factor 3(RUNX3)on the proliferation and migration of Mouse Hepatic Stellate Cells(HSCs),as well as subsequent colla-gen deposition.Methods Mouse hepatic stellate cell line(JS-1)was selected and then morphologically observed and identified under a microscope.After the cells had fully adhered,they were treated with 5 ng/mL of transform-ing growth factor beta 1(TGF-β1)for 24 hours to induce hepatic stellate cell activation.Furthermore,a RUNX3 si-lencing model was established using RUNX3 lentiviral infection.The experiment was divided into four groups:Control group,TGF-β1 group,TGF-β1+siRNA-NC group,and TGF-β1+siRNA-RUNX3 group.Protein expression changes of RUNX3,alpha-smooth muscle actin(α-SMA),and Alpha 1 type I collagen(Collagen I)were detected using Western blot method.Cellular immunofluorescence assays were employed to investigate the deposition changes of α-SMA and RUNX3 in hepatic stellate cells.RT-qPCR was utilized to examine the mRNA expression changes of RUNX3,α-SMA,and Collagen I.The proliferative capacity of hepatic stellate cells was assessed using Edu staining.The migratory ability of hepatic stellate cells was evaluated through wound healing assays and Tran-swell migration experiments.Results Compared with Control group,a significant elevation in RUNX3 was ob-served in the TGF-β1-induced activated HSCs(P<0.01).Meanwhile,the protein and mRNA levels of fibrosis-related markers and α-SMA and Collagen I were significantly upregulated(P<0.001).Additionally,the prolifera-tion and migration capabilities of HSCs were significantly enhanced(P<0.001).In contrast,when compared to TGF-β1+siRNA-NC group,TGF-β1+siRNA-RUNX3 group exhibited a notable decrease in RUNX3 and other re-lated indicators,such as the protein and mRNA levels of α-SMA and Collagen I(P<0.05).Concurrently,the pro-liferation and migration capabilities of HSCs were significantly inhibited in TGF-β1+siRNA-RUNX3 group(P<0.01).Conclusion Silencing RUNX3 can inhibit the deposition of collagen and the proliferation and migration of hepatic stellate cells.Conversely,RUNX3 promotes the proliferation and migration capabilities of HSCs,thereby facilitating the activation of HSC.关键词
RUNX3/肝星状细胞/活化/增殖/迁移/肝纤维化/胶原沉积Key words
RUNX3/hepatic stellate cells/activate/proliferation/migration/hepatic fibrosis/collagen deposi-tion分类
医药卫生引用本文复制引用
凌辉,汪先晨,尤峻柏,范家好,崔笑,沙纪名,余立权..RUNX3对肝星状细胞活化增殖和迁移能力的影响[J].安徽医科大学学报,2026,61(2):277-284,8.基金项目
安徽省自然科学基金项目(编号:1808085MH270) （编号:1808085MH270）
安徽省高校科研计划项目(编号:2024AH050797) （编号:2024AH050797）
合肥市自然科学基金项目(编号:2021038) Fund programs Natural Science Foundation of Anhui Province(No.1808085MH270) （编号:2021038）
Natural Science Re-search Project of Anhui Educational Committee(No.2024AH050797) （No.2024AH050797）
Natural Science Foundation of Hefei(No.2021038) （No.2021038）
