生物资源2026，Vol.48Issue(1)：21-28,8.DOI:10.14188/j.ajsh.20251223002
过表达山豆根StLDC基因对烟草生物碱含量的影响
Effect of overexpression of StLDC gene from Sophora tonkinensis on alkaloid content in tobacco
摘要
Abstract
Lysine decarboxylase(LDC)is the first key enzyme gene in the biosynthetic pathway of quinolizidine al-kaloids.Based on the obtained full-length sequence of StLDC gene from Sophora tonkinensis,an overexpression vector,p BWA(V)HS-StLDC,was constructed.This vector was subsequently introduced into K326 tobacco via Agrobacteri-um-mediated transformation to elucidate the biological function of StLDC.Molecular and metabolic analyses of the ob-tained transgenic plants revealed that qRT-PCR confirmed the successful overexpression of StLDC in tobacco.High per-formance liquid chromatography-electrospray ionization-mass/mass spectrometry(HPLC-ESI-MS/MS)detection indi-cated that the contents of matrine and oxymatrine in transgenic plants were significantly higher than those in wild-type(WT)plants,with the highest matrine content reaching 29.11 times and the highest oxymatrine content reaching 69.43 times that of the WT.These results indicate that the StLDC gene can significantly increase the contents of matrine and oxymatrine in tobacco,providing important genetic resources for the cultivation of plants with high alkaloid content,espe-cially S.tonkinensis.关键词
山豆根/赖氨酸脱羧酶基因/生物碱/功能验证Key words
Sophora tonkinensis Gagnep./lysine decarboxylase gene/alkaloids/functional verification分类
医药卫生引用本文复制引用
梁莹,秦双双,何弘,韦桂丽,梁细妹,韦范..过表达山豆根StLDC基因对烟草生物碱含量的影响[J].生物资源,2026,48(1):21-28,8.基金项目
国家自然科学基金项目(82560744) （82560744）
广西自然科学基金项目(2023GXNSFAA026487) （2023GXNSFAA026487）
