生物资源2026，Vol.48Issue(1)：29-36,8.DOI:10.14188/j.ajsh.20251129001
常见人工养殖胡蜂和西方蜜蜂蜂毒性状比较研究
Venom trait differences between commonly reared wasps and the Western honeybee
摘要
Abstract
In traditional Chinese medicine,honeybee venom and wasp venom are widely used for dispelling wind,detoxifying,combating microbes,reducing inflammation,and relieving swelling and pain.The components of honeybee venom have been well characterized;nevertheless,those of wasp venom remain largely unexplored.Therefore,it is necessary to clarify the compositional differences between wasp venom and honeybee venom.In this study,three wasp species(Vespa basalis Smith,Vespa mandarina Smith and Vespa velutina Lepeletier)and the Western honeybee(Apis melliferia Linnaeus)were selected to investigate their body sizes and venom sac sizes.Liquid chromatography-tandem mass spectrometry(LC-MS/MS)was employed for component analysis to identify venom polypeptides and proteins.Additionally,the contents of amino acids and 5-hydroxytryptamine,as well as the bioactivities of phospholipase and hyal-uronidase,were determined.The results showed that wasps had significantly larger body sizes and venom sac sizes than honeybees,indicating that wasps likely produce greater quantities of venom.Despite the lack of reference data in public databases,which hindered the annotation of many polypeptides and proteins,the overall composition of wasp venom was found to differ from that of honeybee venom.Specifically,notable differences were observed in polypeptides,venom al-lergens,and phospholipases between the two venoms,along with discrepancies in 18 common amino acid contents.Moreover,wasp venom contained a significantly higher level of 5-hydroxytryptamine than honeybee venom.Unexpected-ly,the bioactivities of phospholipase and hyaluronidase in wasp venom were significantly lower than those in honeybee venom.Collectively,the unique compositional profile of wasp venom suggests that further research on its components is necessary,which may help to reveal the potential of wasp venom as a medicinal resource.关键词
蜂毒/多肽和蛋白/磷脂酶A/透明质酸酶/5-羟色胺Key words
bee venom/polypeptide and protein/phospholipase A/hyaluronidase/5-hydroxytryptamine分类
生物科学引用本文复制引用
谢正华,王健敏,徐涓,冯璇璇..常见人工养殖胡蜂和西方蜜蜂蜂毒性状比较研究[J].生物资源,2026,48(1):29-36,8.基金项目
云南省中青年学术和技术带头人后备人才项目(202405AC350077) （202405AC350077）
云南省基础研究计划项目(202401AS070015,202301AT070432) （202401AS070015,202301AT070432）
