生物资源2026，Vol.48Issue(1)：82-91,10.DOI:10.14188/j.ajsh.20250901001
基于高通量测序的太白米内生真菌多样性分析
Diversity analysis of endophytic fungi in Taibaimi(Notholirion bulbuliferum)based on high-throughput sequencing
摘要
Abstract
Taibaimi is a valuable medicinal herb in Shaanxi Province,known for its unique efficacy and high economic value.Over-excavation for a long time has led to serious damage to wild Taibaimi resources,and the yield has been decreasing year by year.Studies have shown that endophytic fungi have potential value in solving the sustainable uti-lization of medicinal plant resources.However,the research on the diversity of endophytic fungi in Taibaimi has not been reported.Endophytic fungi from different tissues of Taibaimi plants collected in the Taibai Mountain region of Shaanxi Province were identified and analyzed using high-throughput sequencing.A total of 1 275 OTU sequences were ob-tained,with the highest number detected in the small bulbs(450),which are considered the medicinal part of the plant,followed by roots(315),leaves(260),and stems exhibiting the lowest count(250).Notable variations were observed in the community structure and abundance of endophytic fungi across different tissues.Beauveria was identified as the dominant genus in both leaf and stem tissues,whereas Aphanocladium dominated in roots,and Aspergillus was the pre-dominant genus in small bulb tissues.LEfSe analysis further revealed the presence of multiple distinct indicator species across all four tissue types,including roots,stems,leaves,and small bulbs.In summary,significant variations exist in the population structure of endophytic fungi in different tissues of the endangered plant Taibaimi,indicating a rich diversi-ty of fungal resources with considerable potential for further development and application.关键词
太白米/内生真菌/高通量测序/多样性/群落结构Key words
Taibaimi[Notholirion bulbuliferum(Lingelish.)Stearn.]/endophytic fungi/high-throughput se-quencing/diversity/community structure分类
生物科学引用本文复制引用
李彤,储天,焦晨莉,杨娟娟,张萌萌,白杨,卫培峰,陈亮亮..基于高通量测序的太白米内生真菌多样性分析[J].生物资源,2026,48(1):82-91,10.基金项目
陕西省教育厅专项科研计划项目(23JK0399) （23JK0399）
陕西省重点研发计划(2025NC-YBXM-058) （2025NC-YBXM-058）
陕西省中医药科技创新提质扩能计划(TZKN-CXPT-03) （TZKN-CXPT-03）
