福建农业学报2026，Vol.41Issue(1)：1-12,12.DOI:10.19303/j.issn.1008-0384.2026.01.001
NF-YC15转录因子调控木薯抗细菌性枯萎病的互作蛋白筛选及功能验证
Screening and Functional Verification of Interacting Proteins of NF-YC15 Transcription Factor in Regulating Resistance to Bacterial Blight of Cassava
摘要
Abstract
[Objective]Molecular mechanism of NF-YC15 transcription factor associated with the regulation of bacterial blight resistance of Manihot esculenta was investigated.[Methods]Yeast two-hybrid was employed to determine the interacting proteins with the MeNF-YC15 transcription factor.Bimolecular fluorescence complementation and pull-down experiments were conducted to verify the interactions.On overexpressed cassava,bacterial blight pathogens were inoculated to study disease resistance of the plant.Through transcriptome sequencing,downstream disease-resistant genes regulated by MeNF-YC15 were identified,and the molecular mechanism analyzed by quantitative PCR.[Results]The key inhibitor of jasmonic acid ZIM domain protein MeJAZ2.2 in the signal transduction pathway was identified to be the sole protein interacting with MeNF-YC15,which was further verified in vivo and in vitro in plants.The bacteria and disease lesion areas on the significantly taller MeNF-YC15-overexpressed plants were significantly smaller than those on the MeNF-YC15-MeJAZ2.2-co-overexpressed counterparts and control.MeNF-YC15 activated the downstream disease resistant WRKY68,matrix metalloproteinase-3(MMP-3),and leucine-rich repeat receptor-like kinases(LRR-RLK)of jasmonic acid,and in contrast,MeJAZ2.2 inhibited the expressions.[Conclusion]MeNF-YC15 and MeJAZ2.2 antagonistically regulated the expression of downstream disease-resistant genes of jasmonic acid and the resistance of cassava plant to the bacterial blight through the jasmonic acid pathway.关键词
木薯/细菌性枯萎病/MeNF-YC15/抗病/茉莉酸Key words
Cassava/bacterial wilt disease/MeNF-YC15/disease resistance/jasmonic acid分类
农业科技引用本文复制引用
刘永敏,杨澜,闫文雷,曾鸿秋..NF-YC15转录因子调控木薯抗细菌性枯萎病的互作蛋白筛选及功能验证[J].福建农业学报,2026,41(1):1-12,12.基金项目
国家自然科学基金项目(32401763) （32401763）
