福建农业学报2026，Vol.41Issue(1)：13-22,10.DOI:10.19303/j.issn.1008-0384.2026.01.002
牡丹花青素合成基因PqMYB113启动子克隆及上游调控因子筛选
Cloning and Upstream Regulatory Factors of Anthocyanin Synthesis PqMYB113 Promoter in Paeonia qiui
摘要
Abstract
[Objective]The PqMYB113 transcription factor is a key gene promoting anthocyanin synthesis in the leaves of Paeonia qiui,but the upstream regulatory mechanism remains unclear.Uncovering the upstream regulatory factors of PqMYB113 will provide theoretical references for revealing the mechanism of anthocyanin synthesis and breeding new varieties of peonies.[Methods]A yeast one-hybrid cDNA library was constructed using the Gateway technology.Promoter of PqMYB113 of P.qiui leaves was cloned and verified.3-amino-1,2,4-triazole(3-AT)was added to the amino acid-deficient medium to self-activate and screen concentration of the promoter.Upstream regulatory factors binding on the promoter were identified using yeast one-hybrid technology.[Results]The self-activation inhibition concentration of PqMYB113 was 90 mmol·L−1 3-AT.The constructed cDNA expression library had a capacity of 5.5×106 CFU·mL−1 and a total clone number of 1.1×107 CFU.Through the yeast one-hybrid experiments,93 upstream regulatory factors of PqMYB113 were screened.They included two transcription factors,one chromatin remodeling factor interaction factor,and one chromatin remodeling factor.The promoter contained environmental response elements on light and stress,hormone response elements on abscisic acid,jasmonic acid,and ethylene,and multiple binding sites for transcription factors,such as MYB and MYC.[Conclusion]A yeast one-hybrid cDNA library for P.qiui was constructed.The functional elements on the promoter in the identified upstream regulatory factors of PqMYB113 were predicted.The results provided for further studies on the regulatory mechanism of anthocyanin synthesis and breeding of superior P.qiui varieties.关键词
卵叶牡丹/PqMYB113/启动子/cDNA文库/酵母单杂交Key words
Paeonia qiui/PqMYB113/promoter/cDNA library/yeast one-hybrid assay分类
农业科技引用本文复制引用
牛迎安,张玥,罗建让..牡丹花青素合成基因PqMYB113启动子克隆及上游调控因子筛选[J].福建农业学报,2026,41(1):13-22,10.基金项目
国家自然科学基金项目(32271950) （32271950）
陕西省科技计划资助项目(2022NY-146) （2022NY-146）
