佛山科学技术学院学报（自然科学版）2026，Vol.44Issue(1)：8-14,7.
猪繁殖与呼吸综合征病毒糖蛋白2a的原核表达及多克隆抗体制备
Prokaryotic expression of glycoprotein 2a of PRRSV and preparation of its polyclonal antibody
摘要
Abstract
To generate polyclonal antibodies against Glycoprotein 2a(GP2a)of Porcine Reproductive and Respiratory Syndrome Virus(PRRSV),the recombinant GP2a protein was expressed in a prokaryotic system using the genome of the highly pathogenic XH-GD strain as a template.The purified recombinant GP2a was used to immunize rabbits to produce rabbit polyclonal antibodies.The antiserum was subsequently evaluated for its titer and specificity.The results showed that the obtained polyclonal antibodies had a titer of 1∶12,800 and could specifically react with eukaryotic-expressed Flag-GP2a.This indicates that the prokaryotically expressed recombinant GP2a protein possesses good immunogenicity,and the resulting antibodies exhibit high titer and desirable specificity.These findings lay a material foundation for further investigation into the function of PRRSV GP2a.关键词
PRRSV/糖蛋白 2a/原核表达/多克隆抗体Key words
PRRSV/GP2a/prokaryotic expression/polyclonal antibody分类
农业科技引用本文复制引用
丘智恒,辛宁,霍展棠,刘栩沂,马春全,陈耀..猪繁殖与呼吸综合征病毒糖蛋白2a的原核表达及多克隆抗体制备[J].佛山科学技术学院学报（自然科学版）,2026,44(1):8-14,7.基金项目
广东省基础与应用基础研究项目(2019A1515110056) （2019A1515110056）
