佛山科学技术学院学报（自然科学版）2026，Vol.44Issue(2)：48-55,8.
农杆菌介导剑叶文心兰遗传转化体系中抗生素的筛选
Screening of antibiotics in the Agrobacterium-mediated genetic transformation system of leaf disc method for Tolumnia
杜俊祎 1周荣2
作者信息
- 1. 佛山大学农业与生物工程学院,广东 佛山 528225||广东省农业科学院环境园艺研究所,广东 广州 510640
- 2. 佛山大学农业与生物工程学院,广东 佛山 528225||佛山大学交通与土木建筑学院,广东 佛山 528225
- 折叠
摘要
Abstract
To optimize the efficient and stable genetic transformation system of Oncidium ensifolium,in vitro leaves were used as explants in this study.The effects of 3 types of selective antibiotics on callus and the efficacy of 2 types of bacteriostatic antibiotics were investigated via concentration gradient assays and mathematical/statistical analyses.Results showed that,as revealed by Duncan's multiple range test and analysis of variance(ANOVA),0.18 mg/L Basta,9 mg/L hygromycin(Hyg),and 100 mg/L kanamycin(Kana)completely inhibited adventitious bud differentiation in leaves(P＜0.01),and thus were identified as the selection pressure for resistant buds.The half-lethal concentrations(LC50)for subcultured seedlings were 0.12 mg/L Basta,5 mg/L Hyg,and 40 mg/L Kana(P＜0.05),which were designated as the screening concentrations for resistant seedlings.O.ensifolium exhibited antibiotic sensitivity in the order of Basta＞Hyg＞Kana(all P＜0.01),indicating that the bar gene is more suitable as a selectable marker.Additionally,100 mg/L cefotaxime(Cef)effectively inhibited Agrobacterium proliferation while maintaining a high leaf survival rate.This study provides critical parameters for optimizing the genetic transformation system and screening transgenic plants in Tolumnia.关键词
剑叶文心兰/遗传转化/农杆菌/抗生素Key words
Tolumnia/genetic transformation/Agrobacterium/antibiotics分类
农业科技引用本文复制引用
杜俊祎,周荣..农杆菌介导剑叶文心兰遗传转化体系中抗生素的筛选[J].佛山科学技术学院学报（自然科学版）,2026,44(2):48-55,8.
