广东医学2026，Vol.47Issue(2)：179-185,7.DOI:10.13820/j.cnki.gdyx.20253317
基于RANKL/OPG通路探讨补肾壮骨方联合机械振动对去卵巢大鼠骨代谢的调控机制
Regulatory mechanism of Bushen Zhuanggu Formula combined with mechanical vibration on bone metabolism in ovariectomized rats via the RANKL/OPG pathway
摘要
Abstract
Objective To investigate the effects of Bushen Zhuanggu Formula combined with mechanical vibra-tion on bone metabolism in ovariectomized(OVX)rats and to elucidate the underlying molecular mechanisms,with a fo-cus on the RANKL/OPG signaling pathway.Methods Forty-eight female Sprague-Dawley rats were randomly as-signed to five groups:sham-operated group,OVX model group,Bushen Zhuanggu Formula group,mechanical vibration group,and combination therapy group.An osteoporosis model was established by bilateral ovariectomy.Vibration parame-ters were optimized using an orthogonal experimental design(frequency 30 Hz,amplitude 2 mm,twice daily for 15 min per session),and the herbal formula was administered orally at a dose of 10 g/kg,followed by vibration training 4 hours post-administration.After 8 weeks of intervention,bone mineral density(BMD)was measured by dual-energy X-ray absorptiometry(DEXA).Serum levels of receptor activator of nuclear factor-κB ligand(RANKL)and osteoprotegerin(OPG)were quantified by ELISA.Bone microarchitecture was evaluated using micro-computed tomography(micro-CT).Quantitative real-time PCR(qPCR)was performed to assess the expression of genes related to the Wnt/β-cate-nin and MAPK/ERK signaling pathways.Results Compared with the OVX model group,femoral BMD in the combina-tion therapy group increased by 34.6％(P＜0.05),which was significantly greater than that observed in either monother-apy group.Serum RANKL levels were reduced by 42.3％,while OPG levels increased by 38.6％,resulting in a marked-ly decreased RANKL/OPG ratio.Micro-CT analysis revealed a 52.7％increase in trabecular number and substantial im-provement in bone microarchitecture.Membrane receptor analysis demonstrated synergistic upregulation of β1-integrin,Piezo1,and estrogen receptor α(ERα)mRNA expression.Pathway analysis further confirmed significant activation of the Wnt/β-catenin pathway(increase of 162.9％)and enhanced MAPK/ERK signaling(increase of 109.5％)in the com-bination therapy group.Conclusion Bushen Zhuanggu Formula combined with mechanical vibration exerts synergistic os-teoprotective effects in ovariectomized rats by coordinately upregulating mechanosensitive and hormone-related membrane receptors,activating multiple bone metabolic signaling pathways,and restoring RANKL/OPG balance.These findings pro-vide experimental evidence supporting an integrative therapeutic strategy for postmenopausal osteoporosis.关键词
补肾壮骨方/机械振动/骨代谢信号通路/骨代谢/去卵巢大鼠Key words
Bushen Zhuanggu Formula/mechanical vibration/bone metabolism signaling pathway/bone metab-olism/ovariectomized rats分类
医药卫生引用本文复制引用
戴杰,杨红,陈艺菲,黄莉,谭新,邓伟民..基于RANKL/OPG通路探讨补肾壮骨方联合机械振动对去卵巢大鼠骨代谢的调控机制[J].广东医学,2026,47(2):179-185,7.基金项目
广州市增城区科技计划项目(2021ZCMS08) （2021ZCMS08）
