广东医学2026，Vol.47Issue(2)：194-200,7.DOI:10.13820/j.cnki.gdyx.20251892
LncRNA ZBED3-AS1靶向miR-506-5p对骨关节炎软骨细胞增殖、分化的影响
LncRNA ZBED3-AS1 Regulates Proliferation and Differentiation of Osteoarthritic Chondrocytes by Targeting miR-506-5p
摘要
Abstract
Objective To investigate the effects of long non-coding RNA ZBED3 antisense RNA 1(lncRNA ZBED3-AS1)targeting microRNA-506-5p(miR-506-5p)on the proliferation and differentiation of osteoarthritic(OA)chondrocytes,and to provide a theoretical basis for the development of novel therapeutic strategies for osteoarthritis.Methods Human chondrocytes(CHON-001)cultured under normal conditions were used as the control group.An OA chondrocyte model was established by interleukin-1β(IL-1β)stimulation and randomly divided into the following groups:Model,OE-NC,OE-lncRNA ZBED3-AS1,OE-lncRNA ZBED3-AS1+pcDNA-NC,and OE-lncRNA ZBED3-AS1+pcDNA-miR-506-5p.The expression levels of lncRNA ZBED3-AS1 and miR-506-5p were de-termined by quantitative real-time PCR(qRT-PCR).The targeting relationship between lncRNA ZBED3-AS1 and miR-506-5p was verified using a dual-luciferase reporter assay.Cell proliferation was assessed by CCK-8 assay,and apoptosis was analyzed by flow cytometry.The mRNA and protein expression levels of proliferating cell nuclear antigen(PCNA),bone morphogenetic protein 2(BMP-2),and Runt-related transcription factor 2(Runx2)were detected by qRT-PCR and Western blotting,respectively.Results Compared with the control group,the model group showed sig-nificantly decreased expression of lncRNA ZBED3-AS1,reduced cell viability,and downregulated mRNA and protein levels of PCNA,BMP-2,and Runx2,while miR-506-5p expression and apoptosis rate were significantly increased(P＜0.05).Compared with the model or OE-NC groups,overexpression of lncRNA ZBED3-AS1 significantly in-creased cell viability and the expression of PCNA,BMP-2,and Runx2,while decreasing miR-506-5p expression and apoptosis rate(P＜0.05).These effects were partially reversed by co-transfection with pcDNA-miR-506-5p.Dual-luciferase reporter assays demonstrated that miR-506-5p significantly reduced luciferase activity in cells transfected with wild-type lncRNA ZBED3-AS1 constructs(P＜0.05),confirming a direct targeting interaction.Conclusion LncRNA ZBED3-AS1 promotes proliferation and differentiation and inhibits apoptosis of osteoarthritic chondrocytes by targeting and suppressing miR-506-5p.This regulatory axis may represent a potential therapeutic target for osteoarthri-tis.关键词
长链非编码RNA ZBED3反义RNA1/微小RNA-506-5p/骨关节炎/软骨细胞增殖/软骨细胞分化Key words
LncRNA ZBED3-AS1/miR-506-5p/osteoarthritis/chondrocyte proliferation/chondrocyte dif-ferentiation分类
医药卫生引用本文复制引用
李炳南,李奎蒙,刘鹏飞..LncRNA ZBED3-AS1靶向miR-506-5p对骨关节炎软骨细胞增殖、分化的影响[J].广东医学,2026,47(2):194-200,7.基金项目
张家口市重点研发计划项目(2021023D) （2021023D）
