广东医学2026，Vol.47Issue(2)：213-222,10.DOI:10.13820/j.cnki.gdyx.20250644
中医湿证的肠道微生态特征的探索性研究
Exploratory study on gut microbiota characteristics in individuals with the traditional Chinese medicine dampness syndrome
摘要
Abstract
Objective To investigate the clinical characteristics,blood biochemical indices,gut microbiota fea-tures,and their interrelationships in individuals with dampness syndrome as defined by traditional Chinese medicine(TCM),and to provide a modern biomedical interpretation of the biological basis of dampness syndrome.Methods Participants undergoing routine health examinations at a health management center were enrolled.TCM dampness syn-drome scores were assessed,and fecal and blood samples were collected.Shotgun metagenomic sequencing was applied to analyze gut microbiota composition,and standard blood biochemical tests were performed.Participants were divided into a dampness syndrome group and a non-dampness syndrome group,and intergroup differences were systematically com-pared.Results A total of 285 participants were included,comprising 144 individuals with dampness syndrome and 141 without.Phenotypic and biochemical analyses revealed that individuals with dampness syndrome tended to be younger and obese.White blood cell count(OR=1.27)and platelet count(OR=1.01)were identified as independent risk factors for dampness syndrome.Gut microbiota analysis demonstrated that Escherichia coli,Klebsiella pneumoniae,and other op-portunistic pathogens were characteristic taxa in the dampness syndrome group,whereas beneficial bacteria such as Bifidobacterium longum and Bifidobacterium adolescentis were enriched in the non-dampness group.A random forest model integrating phenotypic and microbiota data improved the predictive performance for dampness syndrome(AUC=0.769).Mediation analysis further indicated that Ruminococcus_sp_AF13_28 influenced dampness syndrome scores by in-creasing alanine aminotransferase levels.Conclusion The development of TCM dampness syndrome is closely associated with obesity,a state of low-grade inflammation,gut microbiota dysbiosis,and metabolic dysfunction.Gut microbiota may contribute to the occurrence and progression of dampness syndrome by modulating inflammatory and metabolic parameters in the blood.关键词
湿证/肠道菌群/宏基因组学/中医证候Key words
dampness syndrome/gut microbiota/metagenomics/traditional Chinese medicine syndromes分类
医药卫生引用本文复制引用
尚潇潇,孙晨,张伟铮,黄鹂,刘艳敏,邓煜盛,唐文艺,陈洋,何春梅,仇秦威,王成锐,黄唯..中医湿证的肠道微生态特征的探索性研究[J].广东医学,2026,47(2):213-222,10.基金项目
省部共建中医湿证国家重点实验室重点项目(SZ2021ZZ28,SZ2021ZZ31,SZ2021ZZ3002) （SZ2021ZZ28,SZ2021ZZ31,SZ2021ZZ3002）
广东省中医药局科研项目(20244016,20254065) （20244016,20254065）
广州市科技计划项目(2024A03J0732,2024A03J0770) （2024A03J0732,2024A03J0770）
