广东医学2026，Vol.47Issue(2)：235-242,8.DOI:10.13820/j.cnki.gdyx.20251687
布比卡因和罗哌卡因对脐静脉造血干细胞的功能影响
Effects of bupivacaine and ropivacaine on the functional properties of human umbilical vein-derived hematopoi-etic stem cells
摘要
Abstract
Objective To investigate the effects of the commonly used local anesthetics bupivacaine and ropiva-caine on the biological functions of neonatal umbilical cord blood CD34+CD133+hematopoietic stem cells(HSCs),and to provide experimental evidence for evaluating the safety of anesthetic use during stem cell collection and storage.Meth-ods Umbilical cord blood samples were collected from full-term neonates delivered vaginally.CD34+CD133+HSCs were isolated using Ficoll density gradient centrifugation followed by immunomagnetic bead sorting.Cells were exposed to different concentrations(0.1,0.5,and 1 mmol/L)of bupivacaine or ropivacaine.Cell viability was assessed using the CCK-8 assay.Apoptosis was evaluated by Annexin V-FITC/PI double staining with flow cytometry.Intracellular nitric oxide(NO)levels were measured using fluorescent probes combined with flow cytometry.Hematopoietic potential was an-alyzed by methylcellulose colony-forming assays,and cell migratory capacity was evaluated using Transwell migration as-says.Results Compared with the control group.Bupivacaine significantly inhibited cell viability(P=0.027),colony formation,and migratory ability(P=0.006),and increased apoptosis(P=0.008)and intracellular NO levels(P=0.002)in a dose-dependent manner,with particularly pronounced effects at higher concentrations.In contrast,ropiva-caine exerted minimal effects on HSC function at most tested concentrations,showing only mild inhibitory effects on select-ed parameters at the highest concentration and no clear dose-response relationship overall.Apoptosis and NO results sug-gested that bupivacaine may promote HSC apoptosis through the induction of oxidative stress.Conclusion Bupivacaine exhibits dose-dependent cytotoxic effects on neonatal umbilical cord blood CD34+CD133+hematopoietic stem cells,whereas ropivacaine appears relatively safe within the concentration range tested.During obstetric anesthesia,ropivacaine or other local anesthetics with lower cytotoxicity should be preferentially selected to preserve the quality and clinical utility of umbilical cord blood stem cells.关键词
脐静脉造血干细胞/布比卡因/罗哌卡因/细胞毒性Key words
Umbilical vein hematopoietic stem cells/Bupivacaine/Ropivacaine/Cytotoxicity分类
医药卫生引用本文复制引用
李江飞,肖玉花,曹文娟..布比卡因和罗哌卡因对脐静脉造血干细胞的功能影响[J].广东医学,2026,47(2):235-242,8.基金项目
保定市社发类项目(医疗卫生)(2341ZF193) （医疗卫生）
