医学分子生物学杂志2026，Vol.23Issue(2)：127-133,7.DOI:10.3870/j.issn.1672-8009.2026.02.003
七叶皂苷钠改善溃疡性结肠炎大鼠肠黏膜屏障损伤机制研究
Mechanism of Sodium Aescinate in Ameliorating Intestinal Mucosal Barrier Dysfunction in Ulcerative Colitis Rats
摘要
Abstract
Objective To explore the effect of sodium aescinate on intestinal mucosal barrier in rats with ulcerative colitis(UC).Methods A total of 65 SD rats were divided into 6 groups:sham operation group,model group,low-dose,medium-dose and high-dose sodium aescinate groups(2.5,5,10 mg/kg),and positive control group(mesalazine,360 mg/kg),10 rats in each group.UC rat models were induced by DSS.Disease activity index(DAI)scores were recorded af-ter drug intervention.The levels of serum D-lactic acid(D-LA),diamine oxidase(DAO),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and IL-6 were detected.The ulcer area,length and weight of colon,expression levels of myeloperoxidase(MPO),Occludin,zonula oc-cludens-1(ZO-1),nuclear factor-erythroid 2-related factor 2(Nrf-2),antioxidant response ele-ment(ARE)and heme oxygenase-1(HO-1)proteins in colon tissues were compared among dif-ferent groups.HE staining was performed and pathological scores were recorded.Results Medium-dose and high-dose sodium aescinate significantly reduced DAI score and pathological score,allevi-ated colonic ulcers,reduced colon weight and increased colon length(P<0.05).The levels of ser-um D-LA,DAO,TNF-α,IL-1β,IL-6,and colonic MPO were significantly reduced,and the expression of Occludin,ZO-1,p-Nrf2/Nrf2,ARE and HO-1 proteins in colonic tissues was upreg-ulated in the medium-and high-dose sodium aescinate groups(P<0.05).Conclusion Sodium aescinate can ameliorate intestinal mucosal barrier dysfunction in DSS-induced UC rats,and the mechanisms may be related to the inflammation and Nrf-2/ARE/HO-1 signaling pathway.关键词
七叶皂苷钠/溃疡性结肠炎/肠黏膜屏障/Nrf-2/ARE/HO-1信号通路Key words
sodium aescinate/ulcerative colitis/intestinal mucosal barrier/Nrf-2/ARE/HO-1 signaling pathway分类
医药卫生引用本文复制引用
张彤,司梦圆,林莹,王丹,孙吉瑞..七叶皂苷钠改善溃疡性结肠炎大鼠肠黏膜屏障损伤机制研究[J].医学分子生物学杂志,2026,23(2):127-133,7.基金项目
河北省2023年度医学科学研究课题计划项目(No.20232032) This work was supported by a grant from the Hebei Province Medical Science Research Projectin 2023(No.20232032) （No.20232032）
