南方农业学报2026，Vol.57Issue(1)：1-12,12.DOI:10.3969/j.issn.2095-1191.2026.01.001
林下三七—白及轮作缓解连作障碍效应和病原菌交叉侵染分析
Effects of Panax notoginseng(Burk.)F.H.Chen-Bletilla striata rotation cropping under forest on alleviating continuous crop⁃ping obstacles and pathogen cross-infection analysis
摘要
Abstract
[Objective]This study aimed to investigate the effects of Panax notoginseng(Burk.)F.H.Chen-Bletilla striata-Panax notoginseng(Burk.)F.H.Chen rotation cropping under forest on alleviating continuous cropping obstacles of Panax notoginseng(Burk.)F.H.Chen and potential cross-infection risks between pathogens of the two plants,and to evaluate the effects of Panax notoginseng(Burk.)F.H.Chen root-zone soil on growth,quality,and disease of Bletilla striata for elucidating feasibility of Panax notoginseng(Burk.)F.H.Chen-Bletilla striata-Panax notoginseng(Burk.)F.H.Chen rotation cropping,thereby providing a theoretical basis for screening suitable rotation crops that could alleviate continuous cropping obstacles of Panax notoginseng(Burk.)F.H.Chen to achieve sustainable Panax notoginseng(Burk.)F.H.Chen cultivation.[Method]Bletilla striata was cultivated in continuous Panax notoginseng(Burk.)F.H.Chen cropping soil and natural soil under forest,and the differen-ces in growth parameters,quality,and disease of Bletilla striata planted in the two kinds of soil were measured to investigate the effects of continuous Panax notoginseng(Burk.)F.H.Chen cropping soil on Bletilla striata.Continuous Panax notoginseng(Burk.)F.H.Chen cropping soil was further utilized for rotation Bletilla striata cropping,and after that,Panax notoginseng(Burk.)F.H.Chen was replanted in that soil to determine seedling survival rate,dry weight,and saponin content of Panax notoginseng(Burk.)F.H.Chen to evaluate the effects of rotation Bletilla striata cropping on alleviating subsequent continuous cropping obstacles of Panax notoginseng(Burk.)F.H.Chen.Bletilla striata root and leaves were inoculated with pathogens of Panax notogin-seng(Burk.)F.H.Chen root rot and leaves,and Panax notoginseng(Burk.)F.H.Chen root and leaves were inoculated with pathogens of Bletilla striata root rot and leaves respectively,to investigate the cross-infection between pathogens of Panax notoginseng(Burk.)F.H.Chen and Bletilla striata.Real-time fluorescence quantitative PCR was performed to analyze their common cross-infected pathogens.[Result]Compared with natural soil,continuous Panax notoginseng(Burk.)F.H.Chen cropping soil showed no significant negative effects on growth(plant height,biomass,and chloro-phyll content),quality(total phenol,polysaccharide,and Militarine content),or disease(anthracnose,rust,and root rot)of Bletilla striata(P>0.05).Replanting Panax notoginseng(Burk.)F.H.Chen after Panax notoginseng(Burk.)F.H.Chen-Bletilla striata rotation cropping could significantly increase root dry weight of Bletilla striata(P<0.05,the same below),extremely significantly improve seedling survival rate of Bletilla striata(P<0.01,the same below)com-pared with plantation in continuous Panax notoginseng(Burk.)F.H.Chen cropping soil,and extremely significantly im-prove contents of total ginsenosides,Rg1,Rb1,R1,and Rd.Both Panax notoginseng(Burk.)F.H.Chen and Bletilla striata were susceptible to infection by root rot pathogens of Ilyonectria spp.;however,the contents of pathogens of Ilyo-nectria spp.were significantly decreased after rotation Bletilla striata cropping followed by continuous Panax notoginseng(Burk.)F.H.Chen cropping.[Conclusion]Continuous Panax notoginseng(Burk.)F.H.Chen cropping soil under forest exhibits no significant effects on growth,quality,and disease of Bletilla striata;Bletilla striata rotation cropping im-proves Panax notoginseng(Burk.)F.H.Chen seedling survival rate and quality,as well as reduces contents of pathogens of Ilyonectria spp.in soil.Panax notoginseng(Burk.)F.H.Chen-Bletilla striata-Panax notoginseng(Burk.)F.H.Chen rotation cropping is feasible under forest.Root rot pathogens cause cross-infection between Panax notoginseng(Burk.)F.H.Chen and Bletilla striata,therefore continuous monitoring of potential hazards in large-scale cultivation is recom-mended.关键词
三七/连作障碍/白及/轮作/实时荧光定量PCR/交互侵染Key words
Panax notoginseng(Burk.)F.H.Chen/continuous cropping obstacle/Bletilla striata/rotation crop-ping/real-time fluorescence quantitative PCR/cross-infection分类
农业科技引用本文复制引用
江冰冰,郭存武,段生双,李瑞,王正平,侯国喜,朱罗娟,毕玲芳,朱书生,杨敏..林下三七—白及轮作缓解连作障碍效应和病原菌交叉侵染分析[J].南方农业学报,2026,57(1):1-12,12.基金项目
云南省农业基础研究联合专项(202301BD070001-187) （202301BD070001-187）
云南省农林联合重点项目(202101BD070001-003) （202101BD070001-003）
云南省"兴滇英才支持计划"青年人才专项(YDYC-QNRC-2023-0399) （YDYC-QNRC-2023-0399）
云南省重大科技专项(202102AE090042) Yunnan Agriculture Fundamental Research Joint Special Project(202301BD070001-187) （202102AE090042）
Yun-nan Agriculture and Forestry Joint Key Project(202101BD070001-003) （202101BD070001-003）
Young Talent Project of Yunnan Revitalization Talent Support Project(YDYC-QNRC-2023-0399) （YDYC-QNRC-2023-0399）
Yunnan Major Science and Technology Special Project(202102AE090042) （202102AE090042）
