贵州中医药大学学报2026，Vol.48Issue(2)：47-54,8.DOI:10.16588/j.cnki.issn2096-8426.2026.02.009
丹黄散对高糖诱导血管内皮细胞AMPK/mTOR通路及自噬水平的影响
Effects of Danhuang Powder on AMPK/mTOR Pathway and Autophagy Level in Vascular Endothelial Cells Induced by High Glucose
摘要
Abstract
Objective To investigate the protective effect and molecular mechanism of Danhuang Powder a-gainst high glucose-induced vascular endothelial cells injury by regulating the AMPK/mTOR pathway and auto-phagy level.Methods An in vitro model of human umbilical vein endothelial cells was used and divided into a control group(normal culture),a high glucose group(30 mmol/L glucose),a high glucose+growth factor group(30 mmol/L glucose+10%growth factor solution),and a high glucose+Danhuang Powder group(30 mmol/L glucose+10%Danhuang Powder solution).All groups were treated continuously for 24 hours.Cell migration was assessed by wound healing assay.The expression levels of PIK3C3 and Beclin-1 were detected by immunofluorescence method.The expression of key proteins in the AMPK/mTOR pathway and autophagy mark-ers,including LC3A and LC3B,was determined by Western blot.Results Danhuang Powder enhanced cell migration activity under high glucose conditions,promoted the expression of PIK3C3 and Beclin-1 proteins,up-regulated the expression of AMPK,LC3A and LC3B proteins,and down-regulated the expression of mTOR protein.Conclusion Danhuang Powder alleviates high glucose-induced vascular endothelial cells injury by ac-tivating the AMPK/mTOR pathway and regulating autophagy level.关键词
丹黄散/血管内皮细胞/AMPK/mTOR信号通路/自噬Key words
Danhuang Powder/vascular endothelial cells/AMPK/mTOR signaling pathway/autophagy分类
医药卫生引用本文复制引用
杨萱,赵月,张春玲,陈露,邸铁涛,高永跃,史青,赵思思,阎慧敏,向晋..丹黄散对高糖诱导血管内皮细胞AMPK/mTOR通路及自噬水平的影响[J].贵州中医药大学学报,2026,48(2):47-54,8.基金项目
1.国家自然科学基金项目(81960805) （81960805）
2.2025年贵州省基础研究计划(自然科学)面上项目和重点项目(黔科合基础MS[2025]140) （自然科学）
3.贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2025-011) （QZYY-2025-011）
4.贵州中医药大学研究生科研基金项目(YCXKYS2024009). （YCXKYS2024009）
