贵州中医药大学学报2026，Vol.48Issue(2)：59-66,8.DOI:10.16588/j.cnki.issn2096-8426.2026.02.011
基于Plackett-Burman与Box-Behnken设计对丹香葡萄糖滴注液中丹参提取液醇沉工艺研究
A Study on the Alcohol Precipitation Process of Salvia Miltiorrhiza Extract in Danxiang Glucose Infusion Based on Plackett-Burman and Box-Behnken Designs
摘要
Abstract
Objective To optimize the alcohol precipitation process of Salvia miltiorrhiza extract in Danx-iang Glucose infusion.Methods Plackett-Burman design was employed,with the density of water extract,al-cohol concentration during the second alcohol precipitation,and pH value as variables.The comprehensive score of the transfer rates of salvianic acid A sodium and protocatechuic aldehyde was used as the evaluation index.Box-Behnken response surface methodology was applied to optimize the parameters of alcohol precipitation.Re-sults The concentrated aqueous extract of Salvia miltiorrhiza with a relative density of 1.15 was subjected to al-cohol precipitation twice.Firstly,95%ethanol was added to reach 70%alcohol concentration.Secondly,the pH value was adjusted to 7.6 with 10%sodium hydroxide solution,followed by adding 95%ethanol to 77%alcohol concentration.Each precipitation step was stored at 4℃for 12 h under refrigeration.The average transfer rates of salvianic acid A sodium and protocatechuic aldehyde were 58.66%and 78.27%respectively,with an average comprehensive score of 68.46.Conclusions The optimized alcohol precipitation process is stable and reliable,and it can be applied to the alcohol precipitation procedure of Danxiang Glucose Infusion.关键词
丹香葡萄糖滴注液/Plackett-Burman设计/Box-Behnken设计/醇沉工艺Key words
Danxiang Glucose infusion/Plackett-Burman design/Box-Behnken design/alcohol precipita-tion process分类
医药卫生引用本文复制引用
杨燕,杨应勇,罗绍,周威,陈登健..基于Plackett-Burman与Box-Behnken设计对丹香葡萄糖滴注液中丹参提取液醇沉工艺研究[J].贵州中医药大学学报,2026,48(2):59-66,8.基金项目
神奇制药产业化重大研究专项项目(SQ-20210801). （SQ-20210801）
