军事医学2026，Vol.50Issue(2)：130-135,6.DOI:10.7644/j.issn.1674-9960.2025-00157
基于诱导性多能干细胞的人源肠道类器官构建策略及其应用研究进展
Research advances in construction and applications of human intestinal organoids derived from induced pluripotent stem cells
摘要
Abstract
Animal models of intestinal diseases and two-dimensional intestinal tissue cell cultures have served as essential research tools for investigating intestinal diseases.Unlike conventional animal models for intestinal diseases,human intestinal organoids(HIOs)can lessen differences in species-specific backgrounds,costs of experiments and ethical concerns associated with animal experimentation.Human intestinal organoids have intestinal crypts and villus structures,which can simulate the structures of human intestinal tissues more realistically than simple cell culture systems.This review outlines the development of human intestinal organoids in general and methodological systems for inducing and differentiating human induced pluripotent stem cells(hiPSCs)into human intestinal organoids in particular.It also describes the latest advancements in applications of human intestinal organoids in terms of their roles in disease modeling,drug screening and evaluation,and tissue regeneration.An in-depth study on hiPSC-derived HIOs will not only facilitate innovative research into intestinal biology,but also offer a more biomimetic and ethically viable model platform for mechanistic studies on related diseases and clinical translation.关键词
类器官/人源肠道类器官/人诱导多能干细胞Key words
organoids/human intestinal organoids/human induced pluripotent stem cells分类
生物科学引用本文复制引用
方佳瑞,李晓彤,王洪伟,朱恒..基于诱导性多能干细胞的人源肠道类器官构建策略及其应用研究进展[J].军事医学,2026,50(2):130-135,6.基金项目
国家自然科学基金项目(82372373) （82372373）
