辽宁中医药大学学报2026，Vol.28Issue(4)：29-34,6.DOI:10.13194/j.issn.1673-842X.2026.04.005
姜黄素通过调控TXNIP-NLRP3信号通路缓解骨关节炎症损伤与细胞凋亡
Curcumin Alleviates Inflammatory Damage and Cell Apoptosis in Osteoarthritis by Modulating the TXNIP-NLRP3 Signaling Pathway
摘要
Abstract
Objective This study aimed to investigate the protective effects of curcumin on lipopolysaccharide(LPS)-induced osteoarthritic inflammatory damage and its underlying molecular mechanisms.Methods Primary murine articular chondrocytes were cultured in vitro and pretreated with varying concentrations of curcumin(10,20,and 40 µmol/L)before LPS stimulaltion to establish an inflammation-induced injury model.The levels of inflammatory cytokines IL-1β,IL-18,and TNF-α in the cell supernatant were measured using enzyme-linked immunosorbent assay(ELISA).Western blot analysis was performed to assess the protein expression levels of TXNIP,NLRP3,and Caspase-1.The number of ASC specks and the apoptosis rate of chondrocytes were observed using confocal microscopy.Additionally,a murine osteoarthritis model was established via sodium iodoacetate(MIA)induction,and real-time quantitative PCR(qPCR)was utilized to evaluate the protective effects of curcumin on articular cartilage.Results Curcumin inhibited LPS-induced inflammatory cytokine secretion in a dose-dependent manner.Mechanistically,curcumin was found to bind to TXNIP,thereby suppressing the activation of the NLRP3 inflammasome,leading to a reduction in ASC speck formation and chondrocyte apoptosis.In vivo experiments demonstrated that intraperitoneal administration of curcumin(20 mg/kg)alleviated knee joint inflammation in osteoarthritic mice.Conclusion Curcumin mitigates osteoarthritic inflammation and chondrocyte apoptosis by inhibiting the activation of the TXNIP-NLRP3 signaling pathway,suggesting its potential as a therapeutic target for osteoarthritis treatment.关键词
姜黄素/骨关节炎/TXNIP/NLRP3炎症小体/细胞凋亡Key words
curcumin/osteoarthritis/TXNIP/NLRP3 inflammasome/apoptosis分类
医药卫生引用本文复制引用
陶韬,程洋,徐子卿,章春生,周中..姜黄素通过调控TXNIP-NLRP3信号通路缓解骨关节炎症损伤与细胞凋亡[J].辽宁中医药大学学报,2026,28(4):29-34,6.基金项目
江苏省"六大人才高峰"资助项目(2015-WSN-009) （2015-WSN-009）
江苏省卫生健康委员会科研资助项目(H2019099) （H2019099）
