辽宁中医药大学学报2026，Vol.28Issue(4)：35-42,8.DOI:10.13194/j.issn.1673-842X.2026.04.006
探讨电针对颅脑损伤大鼠认知功能及脑组织相关蛋白p-TSC2、p-p70S6K表达影响
Exploration of the Effect of Electroacupuncture on Cognitive Function and Brain Tissue-related Protein p-TSC2 and p-p70S6K Expression in Rats with Brain Injury
摘要
Abstract
Objective This study is to explore the effects of electroacupuncture on phosphorylated tuberous sclerosis complex 2(p-TSC2)and phosphorylated p70 ribosomal protein S6 kinase(p-p70S6K)protein in brain tissue in traumatic brain injury(TBI).Methods Randomly divide SD male rats into a control group,a sham operation group,a model group,and an electroacupuncture group.Model groups were model-prepared by the Fenney strike method.The electroacupuncture group selected"'Baihui'(DU20),'Guanyuan'(CV4),'Quchi'(LI11),'Hegu'(LI4),'Zusanli'(ST36),'Yongquan'(KI1)",and the blank group and sham group did not do other treatment.Electroacupuncture intervention was performed 24 h after mold creation,with density wave,frequency 1 Hz,intensity 1 mA,1 time/d,15 min/time,and continuous treatment for 14 d.The effect of electroacupuncture intervention on cognitive function of TBI rats by the Morris water maze,the brain tissue at three time points after staining(day 3,day 7,and day 14),and immunofluorescent staining and Western Blot(WB)to test the protein expression of p-TSC2 and p-p70S6K in the traumatic brain tissue of TBI model.Results Four groups of SD rats were tested for escape latency immediately before mold creation and on day 3,7 and 14 after electroacupuncture intervention.Compared to the model group,the escape latency period in the electroacupuncture group decreased gradually at the three time points after the intervention(P<0.05).Electroacupuncture intervention on the 3rd day,the model group showed inflammatory cell infiltration,visible massive cerebral tissue exudate,tissue vacuolation changes,significant nuclear pyknosis and karyorrhexis,the electroacupuncture group was superior to the model group.Electroacupuncture intervention on day 7,day 14,inflammatory cells were decreased and more normal neurons were increased compared with the model group.At the three time points after electroacupuncture intervention,the relative expression of p-p70S6K protein was up-regulated(P<0.05)and p-TSC2 protein compared with the model group(P<0.05).Conclusion Electroacupuncture intervention may mediate cellular autophagy by reducing p-TSC2 and increasing p-p70S6K protein expression in the brain tissue of TBI rats,thus promoting the recovery of cognitive function and the protective effect on the brain tissue.关键词
TBI/电针/AMPK/mTOR通路/磷酸化结节性硬化复合物2/磷酸化p70核糖体蛋白S6激酶/自噬Key words
TBI/electroacupuncture/AMPK/mTOR pathway/phosphorylated tuberous sclerosis complex 2/phosphorylated p70 ribosomal protein S6 kinase/autophagy分类
医药卫生引用本文复制引用
王若璇,吴涛,夏曾彩,黄威..探讨电针对颅脑损伤大鼠认知功能及脑组织相关蛋白p-TSC2、p-p70S6K表达影响[J].辽宁中医药大学学报,2026,28(4):35-42,8.基金项目
国家自然科学基金项目(81904310,2025JC-YBMS-894) （81904310,2025JC-YBMS-894）
陕西省自然科学基金青年项目(2021JQ-729) （2021JQ-729）
