量子电子学报2026，Vol.43Issue(2)：266-274,9.DOI:10.3969/j.issn.1007-5461.2026.02.009
多种荧光物质重叠荧光光谱特征提取方法
Extraction method of overlapping fluorescence spectral features of various fluorescent substances
摘要
Abstract
Skin autofluorescence arises from the superposition of various endogenous fluorophores in tissues upon excitation,serving as an indicator of related disease states.However,spectral overlap among endogenous fluorophores complicates the detection of specific targets,so selecting optimal detection wavelengths can enhance the identification effectiveness of these targets.Advanced glycation end products(AGEs),reduced niacinamide adenine dinucleotide(NADH),flavin adenine dinucleotide(FAD)and collagen(COL)were selected as experimental subjects in this work.Firstly,a database of overlapping three-dimensional fluorescence spectra of the four fluorescent substances were constructed.Then,the characteristic regions of the four fluorescent substances were identified using the region integration method,and 178 characteristic wavelengths were selected through the elimination of uninformative variables.Finally,a calibration curve was established based on multiple linear regression,and the recovery rates of each fluorescent substance were calculated.The results showed that the recovery rates of AGEs,NADH,FAD,and COL were 70%-110%,80%-110%,70%-130%,and 50%-190%,respectively,and the predictive accuracy for AGEs,NADH,and FAD of the model based on 178 characteristic wavelengths was acceptable,achieving highly selective screening of detection wavelengths for the three fluorescent substances in overlapping spectra.关键词
生物医学光学/特征荧光/三维荧光光谱/晚期糖基化终末产物Key words
biomedical optics/characteristic fluorescence/three-dimensional fluorescence spectrum/advanced glycation end products分类
医药卫生引用本文复制引用
朱国庆,王贻坤,刘勇,杨瑞芳,张洋..多种荧光物质重叠荧光光谱特征提取方法[J].量子电子学报,2026,43(2):266-274,9.基金项目
国家自然科学基金(62205345),中国科学院合肥物质科学研究院院长基金(YZJJZX202009,YZJJ2021GCH04),安徽省药品监督管理局科技创新项目(AHYJ-KJ-202115),安徽省科技重大专项(202103a07020008),皖江新兴产业技术发展中心项目(WJ21CYHXM07) （62205345）
