南京中医药大学学报2026，Vol.42Issue(3)：365-375,11.DOI:10.14148/j.issn.1672-0482.2026.0365
整合转录组学及网络药理学研究仙连解毒方干预结直肠"炎-癌转化"的机制
Mechanistic Analysis of Xianlian Jiedu Decoction in Inflammation-Associated Colorectal Carcinogenesis Based on Transcriptomics and Network Pharmacology
摘要
Abstract
OBJECTIVE To investigate the therapeutic effects of Xianlian Jiedu Decoction(XLJDD)on colitis-associated colorectal cancer(CAC)and to explore its potential underlying mechanisms.METHODS An azoxymethane/dextran sulfate sodium(AOM/DSS)-induced CAC mouse model was established.UPLC-Q-TOF/MS was employed to identify prototype compounds present in colonic tissue after XLJDD administration.Network pharmacology analysis,molecular docking,transcriptomic sequencing,and valida‑tion of key pathway-related genes were further conducted to systematically elucidate the intervention effects and potential mechanisms of XLJDD against CAC.RESULTS In the AOM/DSS-induced CAC mouse model,XLJDD treatment markedly improved tumor-associated pathological features,as evidenced by reduced tumor burden,restored colon length,and alleviated disease activity index(DAI).UPLC-Q-TOF/MS analysis identified 22 exogenous compounds derived from XLJDD in colonic tissue.In addition,XLJDD sig‑nificantly downregulated the expression of multiple inflammatory cytokines while upregulating tight junction-related genes in the co‑lon.Transcriptomic analysis revealed that XLJDD treatment enhanced overall PPAR signaling pathway activity while suppressing the Wnt signaling pathway.Consistently,the expression levels of PPAR pathway-related genes Pparα,Cpt1a,and Ehhadh were upregu‑lated following XLJDD intervention(P<0.05,P<0.01),whereas the Wnt pathway-related genes Axin2 and Lef1 were significantly downregulated(P<0.05,P<0.01).CONCLUSION XLJDD may intervene in inflammation-associated colorectal carcinogenesis by activating the PPAR signaling pathway and suppressing the Wnt signaling pathway.关键词
仙连解毒方/结肠炎相关性结直肠癌/UPLC-Q-TOF/MS/转录组/PPAR信号通路/Wnt信号通路Key words
Xianlian Jiedu Decoction/colitis-associated colorectal cancer/UPLC-Q-TOF/MS/transcriptomics/PPAR signal‑ing pathway/Wnt signaling pathway分类
医药卫生引用本文复制引用
罗婧雯,徐玮铖,闫秋莹,陶李蕙苹,康安,程海波,吴其标,孙东东..整合转录组学及网络药理学研究仙连解毒方干预结直肠"炎-癌转化"的机制[J].南京中医药大学学报,2026,42(3):365-375,11.基金项目
国家自然科学基金面上项目(82374287) （82374287）
国家自然科学基金组织间合作研究项目(82361168663) （82361168663）
江苏省中医药科技发展计划重点项目(ZD202201) （ZD202201）
