南京中医药大学学报2026，Vol.42Issue(3)：408-416,9.DOI:10.14148/j.issn.1672-0482.2026.0408
基于蒲公英来源纳米纤维素的载氧凝胶制备与细胞活性研究
Preparation and Cellular Activity Study of Oxygen-Carrying Gel Based on Taraxaci Herba-Derived Cellu‑lose Nanofibrils
摘要
Abstract
OBJECTIVE To prepare an oxygen-loaded gel based on Taraxaci Herba-derived cellulose nanofibrils and investi‑gate its activity in promoting cell proliferation,migration,and angiogenesis.METHODS Three types of oxidized cellulose nanofibers(CNF--1,CNF--2,CNF--3)with different degrees of substitution were synthesized via the TEMPO-mediated oxidation method.The metal ion species was determined by evaluating the cytotoxicity,cell proliferation-promoting effects,and gel-forming ability with oxi‑dized cellulose nanofibrils.The optimal preparation conditions for the oxidized cellulose nanofibril gel were established,and oxygen nanobubbles were incorporated into the gel to assess its cellular activity.RESULTS CNF--3 contained the most carboxyl groups and exhibited the fastest gelation rate.As a concentration of 2.0%,it can quickly form a stable gel.Zn2+demonstrated low cytotoxicity,strong proliferative activity,and favorable gel-forming performance.The CNF--3 gel crosslinked with Zn²⁺ effectively encapsulated oxygen nanobubbles and exhibited favorable activity in promoting cell proliferation,migration,and angiogenesis.CONCLUSION Ta-raxaci Herba-derived cellulose nanofibers can be crosslinked into a gel via metal ions and serve as a carrier for oxygen nanobubbles,showing potential for applications in wound healing and related treatments.关键词
蒲公英/氧化纳米纤维素/水凝胶/氧纳米气泡/金属离子Key words
Taraxaci Herba/oxidized cellulose nanofibers/hydrogel/oxygen nanobubbles/metal ions分类
医药卫生引用本文复制引用
田书宁,姚树佳,乔宏志..基于蒲公英来源纳米纤维素的载氧凝胶制备与细胞活性研究[J].南京中医药大学学报,2026,42(3):408-416,9.基金项目
国家自然科学基金面上项目(82274220) （82274220）
南京中医药大学一流学科"引领计划"(ZYXYL2024-012) （ZYXYL2024-012）
江苏省高等学校基础科学研究重大项目(25KJA360004) （25KJA360004）
