热带作物学报2026，Vol.47Issue(3)：588-602,15.DOI:10.3969/j.issn.1000-2561.2026.03.004
荔枝LcGAMYB基因家族鉴定及矮化相关分子标记开发
Identification of the LcGAMYB Gene Family in Litchi chinensis and Development of Molecular Markers Associated with Dwarfing
摘要
Abstract
Dwarfism is a key trait for Litchi chinensis breeding and cultivation.Focusing on the candidate gene LcGAMYB23 implicated in dwarfism by prior quantitative trait locus(QTL)mapping,we evaluated the MYB transcription factor family background and its molecular application potential.A genome-wide bioinformatic survey identified 151 members of the LcMYB gene family.Conserved motif and domain analyses showed that all family members harbored the canonical Myb_DNA-binding domain,indicating high sequence conservation in litchi.We further cloned and sequenced LcGAMYB23 from three standard(non-dwarf)cultivars(9918,Feizixiao,FZX;Sanyuehong,SYH)and three dwarf culti-vars(Nuomici,NMC;Yamulong,YML;Ziniangxi,ZNX),and detected consistent sequence differences between the two groups.Based on the divergent single-nucleotide polymorphism(SNP)sites,we developed molecular markers and vali-dated them across 70 cultivars.The markers effectively discriminated standard versus dwarf types at the cDNA level,with concordant results at the genomic DNA level.The findings would provide practical tools for molecular typing and marker-assisted selection of dwarfism in litchi and lay the groundwork for functional elucidation of LcGAMYB23.关键词
荔枝/LcGAMYB23/基因家族/生物信息学分析/分子标记Key words
lychee/LcGAMYB23/gene family/bioinformatics analysis/molecular marker分类
农业科技引用本文复制引用
符书英,杨明超,陈丁,何玉坤,邓海达,陈哲,严婷婷,王祥和,胡福初..荔枝LcGAMYB基因家族鉴定及矮化相关分子标记开发[J].热带作物学报,2026,47(3):588-602,15.基金项目
国家自然科学基金项目(No.32260743) （No.32260743）
海南省科技人才创新项目(No.KJRC2023C17) （No.KJRC2023C17）
国家荔枝龙眼产业技术体系项目(No.CARS-32-21). （No.CARS-32-21）
