水产学报2026，Vol.50Issue(4)：41-54,14.DOI:10.11964/jfc.20260115362
鳀FABP基因家族鉴定、进化、表达分析及其生态适应策略
Identification,evolution,expression pattern and ecological adaptation strategies of the FABP gene family in Japanese anchovy(Engraulis japonicus)
摘要
Abstract
The Japanese anchovy(Engraulis japonicus),a keystone lipid-rich species in the Yellow and East China Seas eco-systems,plays a vital role in energy flow,and its lipid metabolism mechanisms significantly influence population dynamics.The fatty acid-binding protein(FABP)gene family is central to lipid metabolism regulation.This study aimed to systematically identify the FABP gene family members in E.japonicus and analyze their molecular characteristics and expression profiles,thereby providing a foundation for elucidating the molecular mechanisms of lipid metabolism and ecological adaptation in this species.Based on the chromosome-level genome of the E.japonicus,FABP gene family members were screened using Hidden Markov Model(HMM)and homology BLAST searches.Their phylogenetic relationships,gene structures,conserved motifs,chromosomal locations,gene duplication events,and three-dimensional protein structures were analyzed through bioinformat-ics approaches.Transcriptome data from different tissues(heart,liver,muscle,gonad),sexes,and body length groups were util-ized to analyze gene expression profiles.A total of 14 FABP genes belonging to 7 subtypes(FABP1,FABP2,FABP3,FABP4,FABP6,FABP7,FABP10a)were identified in the E.japonicus genome,unevenly distributed across 9 chromosomes.Phylogen-etic analysis classified these genes into three distinct clades.Synteny and tandem duplication analyses revealed that FABP1 genes(FABP1.1 and FABP1.2)originated from segmental duplication,while four FABP3 genes(FABP3.1-3.4)formed a tan-dem duplication cluster on chromosome 9.All FABP proteins possess the typical β-barrel tertiary structure,although some FABP7 genes showed deletions of conserved motifs.Transcriptome analysis demonstrated that FABP gene expression exhib-ited significant tissue specificity(e.g.,high expression of FABP1 and FABP10a in liver),sex specificity(e.g.,specific high expression of FABP7 in testis),and certain correlations with body length.Notably,multi-copy genes(e.g.,different paralogs of FABP3 and FABP7)showed markedly divergent expression patterns across tissues,suggesting potential functional differenti-ation.This study presents the first systematic identification and characterization of the FABP gene family in the E.japonicus.Gene duplication events,particularly the tandem duplication of FABP3,and the subsequent expression divergence may enhance the capacity for lipid uptake,transport,and utilization in E.japonicus,representing an adaptive molecular evolution in response to its high-lipid diet and periodic migratory life history.These findings provide novel theoretical insights for understanding the pivotal role of E.japonicus in energy flow within marine ecosystems at the molecular level and for the sustainable utilization of fishery resources.关键词
鳀/脂肪代谢/FABP基因家族/分子特征/表达模式Key words
Engraulis japonicus/lipid metabolism/FABP gene family/molecular characterization/expression pattern分类
农业科技引用本文复制引用
王瑞祥,王焕,柳淑芳,庄志猛..鳀FABP基因家族鉴定、进化、表达分析及其生态适应策略[J].水产学报,2026,50(4):41-54,14.基金项目
国家重点研发计划(2024YFD2400401) （2024YFD2400401）
国家现代农业产业技术体系(CARS-47) （CARS-47）
中央级公益性科研院所基本科研业务费专项(20603022024023) National Key R&D Program of China(2024YFD2400401) （20603022024023）
National Modern Agricultural Industry Techno-logy System(CARS-47) （CARS-47）
Central Public-interest Scientific Institution Basal Research Fund,YSFRI,CAFS(20603022024023) （20603022024023）
