水产学报2026，Vol.50Issue(4)：161-175,15.DOI:10.11964/jfc.20251215256
基于形态和DNA条形码相结合的大量鱼卵群落分析方法及其在琼东海草床-珊瑚礁景观中的应用
A combined morphological and DNA barcoding approach for large-scale analysis of fish egg communities and its application in the seagrass bed-coral reef seascapes of eastern Hainan
摘要
Abstract
This study analyzed the species composition and community structure of fish eggs in a specific seagrass bed–coral reef habitat in eastern Hainan,and evaluated its function as a spawning ground,providing valuable information for fisheries resource management and conservation.Fish eggs were identified using an integrative approach combining DNA barcoding and morphology.A workflow for determining species composition and abundance in large egg-sample datasets based on morpholo-gical–molecular integration was established:① fish eggs were assigned to distinct morphological groups and additional sub-groups according to typical morphological characteristics and subtle differences;② each morphological group/subgroup was counted and subsampled,and each subsampled egg was photographed to record morphological traits and then molecularly sequenced to determine species identity.Species composition and abundance of the community were determined by integrating the above results.A total of 96 fish-egg samples(86 596 eggs)were collected from the nearshore seagrass bed-coral reef sea-scape off Wenchang,Hainan,during April–May 2024,and 20 morphological groups were delineated.COI sequences were suc-cessfully obtained from 1 208 eggs,identifying 66 species belonging to 8 orders,29 families and 45 genera.Perciformes had the highest species richness(30 species,45.5%),followed by Scorpaeniformes(13 species,19.7%).Scorpaenidae contained the most species(11 species,16.7%),followed by Labridae(8 species,12.1%).The correspondence between morphological groups and taxonomic groups was further established.These results confirm the seagrass bed–coral reef seascape as an important spawning habitat for these fishes.This study provides a practical and scalable framework for community-level analyses of large fish-egg datasets,offering a robust methodological foundation for fisheries resource assessment and marine ecological conser-vation.关键词
鱼卵/DNA条形码/海草床-珊瑚礁/琼东Key words
fish eggs/DNA barcoding/seagrass bed-coral reef/eastern Hainan分类
农业科技引用本文复制引用
刘洋豪,苗中博,陆颖,张陆,夏文彤,谢松光..基于形态和DNA条形码相结合的大量鱼卵群落分析方法及其在琼东海草床-珊瑚礁景观中的应用[J].水产学报,2026,50(4):161-175,15.基金项目
国家重点研发计划(2024YFD2401801) （2024YFD2401801）
国家自然科学基金(42166004,42376114) （42166004,42376114）
海南省重点研发专项(ZDYF2024SHFZ070) （ZDYF2024SHFZ070）
海南省热带水生生物技术重点实验室开放课题(SWJS202403) National Key Research and Development Program of China(2024YFD 2401801) （SWJS202403）
National Natural Science Foundation of China(42166004,42376114) （42166004,42376114）
Key Research and Development Program of Hainan Province(ZDYF2024SHFZ070) （ZDYF2024SHFZ070）
Open Project of Hainan Provincial Key Laboratory of Tropical Hydrobiology Technology(SWJS202403) （SWJS202403）
