生命科学研究2026，Vol.30Issue(1)：1-9,9.DOI:10.16605/j.cnki.1007-7847.2025.01.0112
香叶木素通过增强PIK3R1 mRNA稳定性抑制乳腺癌细胞侵袭和迁移
Diosmetin Inhibits the Invasion and Migration of Breast Cancer Cells by Enhancing the Stability of PIK3R1 mRNA
摘要
Abstract
To explore the inhibitory effect and molecular mechanism of diosmetin on the invasion and mi-gration of breast cancer cells,MCF7 and MDA-MB-231 cells were treated with different doses(5 mg/L,10 mg/L,20 mg/L)of diosmetin for 24 h,and then the changes in the invasion and migration abilities of breast cancer cells were detected using the Transwell assay and cell scratch healing assay.The effect of diosmetin on the expression of metastasis-related proteins was further determined by Western-blot.The Gene Expression Om-nibus(GEO)database was used to analyze differentially expressed genes in breast cancer tissues,and the HERB database was used to obtain the regulatory targets of diosmetin.The effect of diosmetin on the ex-pression of phosphatidylinositol 3-kinase,regulatory subunit 1(PIK3R1)was confirmed by real-time fluores-cent quantitative PCR(qRT-PCR)and Western-blot experiments.Finally,actinomycin D was used to deter-mine the effect of diosmetin on the stability of PIK3R1 mRNA.The results showed that diosmetin signifi-cantly inhibited the invasion and migration ability of breast cancer cells in a dose-dependent manner(P＜0.05).Furthermore,in a dose-dependent manner,diosmetin significantly increased the levels of E-cadherin and significantly decreased the levels of N-cadherin and matrix metallopeptidase 2(MMP2).Additionally,PIK3R1 was both a differentially expressed gene in breast cancer and a target of diosmetin.Breast cancer patients with low levels of PIK3R1 had a worse disease-free survival(P＜0.05).Treatment with diosmetin significantly up-regulated the levels of PIK3R1 mRNA and protein(P＜0.05),and also significantly improved the stability of PIK3R1 mRNA.These results indicate that diosmetin can inhibit the invasion and migration of breast cancer cells,which may be achieved by increasing the stability of PIK3R1 mRNA.关键词
香叶木素/磷脂酰肌醇3-激酶调节亚基1(PIK3R1)/乳腺癌/侵袭/迁移Key words
diosmetin/phosphatidylinositol 3-kinase,regulatory subunit 1(PIK3R1)/breast cancer/invasion/migration分类
生物科学引用本文复制引用
李游,叶江南,刘明耀..香叶木素通过增强PIK3R1 mRNA稳定性抑制乳腺癌细胞侵袭和迁移[J].生命科学研究,2026,30(1):1-9,9.基金项目
国家自然科学基金重点项目(81830083) （81830083）
