生命科学研究2026，Vol.30Issue(1)：23-32,10.DOI:10.16605/j.cnki.1007-7847.2025.05.0147
肺炎克雷伯菌肝脓肿继发血流感染患者NK细胞免疫表型与功能特征研究
Immunophenotypic and Functional Characteristics of Natural Killer Cells in Secondary Bloodstream Infection of Klebsiella pneumoniae Liver Abscess
摘要
Abstract
To analyze the immunophenotypic and functional characteristics of natural killer(NK)cells in the peripheral blood of patients with secondary bloodstream infection of Klebsiella pneumoniae liver abscess(KPLABSI),and to explore the role of NK cells in the immune response to infection as well as the impact of diabetes mellitus on NK cells,this study included 18 patients diagnosed with KPLABSI at Peking University Third Hospital during the period October 2022 to May 2024.Blood samples and clinical data were collected from the patients,and flow cytometry was used to detect the immunophenotypes and functional markers of NK cells.Blood samples from 10 healthy individuals collected during the same period served as controls.Correlations between NK cell indicators and laboratory test results were analyzed,and differences between KPLABSI patients with and without diabetes mellitus were compared.The results showed that KPLABSI pa-tients exhibited significant hyperinflammatory responses,liver parenchymal damage,and impaired liver syn-thetic function.Meanwhile,NK cells in these patients exhibited remarkable immunophenotypic and func-tional changes:CD158e expression was significantly lower than that in the healthy control group(P＜0.000 1),and perforin(PFN)expression was significantly reduced in KPLABSI patients with diabetes mellitus compared with those without(P=0.023).Further correlation analysis revealed that NK cells are involved in the complex immune-metabolic interactions during the occurrence and development of KPLABSI.This study preliminarily suggests that NK cells play a crucial role in the anti-infective immunity among KPLABSI patients,and that hyperglycemia promotes the occurrence and progression of KPLABSI by affecting the expression level of PFN in NK cells.关键词
自然杀伤细胞/肺炎克雷伯菌肝脓肿/继发血流感染/糖尿病/固有免疫Key words
natural killer cell/Klebsiella pneumoniae liver abscess/secondary bloodstream infection/dia-betes mellitus/innate immunity分类
生物科学引用本文复制引用
韩忠玉,张吟眉,吴振超,周子鹤,郑佳佳..肺炎克雷伯菌肝脓肿继发血流感染患者NK细胞免疫表型与功能特征研究[J].生命科学研究,2026,30(1):23-32,10.基金项目
国家重点研发计划项目(2022YFC2303200) （2022YFC2303200）
北京市自然科学基金交叉融合非共识创新项目(F252052) （F252052）
北京大学第三医院交叉联合专项(BYSYJC2023005) （BYSYJC2023005）
