生命科学研究2026，Vol.30Issue(1)：63-72,10.DOI:10.16605/j.cnki.1007-7847.2025.05.0146
肝素结合蛋白快速检测方法的建立
Development of a Rapid Detection Method for Heparin-binding Protein
摘要
Abstract
Sepsis is a systemic inflammatory response syndrome caused by infection.It is often complicated by organ dysfunction,which is a major contributor to mortality of patients with severe infections.Heparin-binding protein(HBP),a multifunctional mediator derived from neutrophils,exhibits levels closely correlated with the severity of sepsis and has great potential as a biomarker for early diagnosis and prognosis of sepsis.Herein,after vector construction and prokaryotic expression,the purified recombinant HBP was used as an immunogen to immunize mice.Following this,four stable cell lines secreting anti-HBP monoclonal antibodies were selected through cell fusion technology.The properties of the antibodies were analyzed by indirect en-zyme-linked immunosorbent assay(ELISA).Finally,a colloidal gold immunochromatographic assay for HBP was established using mAb 11 as the labeling antibody and mAb 18 as the capture antibody.This study suc-cessfully generated monoclonal antibodies against HBP using the recombinant HBP,and preliminarily estab-lished a rapid detection method for HBP.This method is of significant importance in resource-limited areas or scenarios where rapid assessment of infection severity is required.关键词
感染/脓毒症/肝素结合蛋白(HBP)/原核表达/单克隆抗体(mAb)/胶体金免疫层析法Key words
infection/sepsis/heparin-binding protein(HBP)/prokaryotic expression/monoclonal antibody(mAb)/colloidal gold immunochromatography分类
生物科学引用本文复制引用
曾晓琪,张鑫兰,胡锦莲,徐叶,王睿,夏川,张玲,刘如石..肝素结合蛋白快速检测方法的建立[J].生命科学研究,2026,30(1):63-72,10.基金项目
湖南省卫生健康委科研计划项目(B202311009257) （B202311009257）
湖南省重点研发计划项目(2019SK2042) （2019SK2042）
