实用医学杂志2026，Vol.42Issue(6)：959-968,10.DOI:10.3969/j.issn.1006-5725.2026.06.008
具有pH响应性载硼替佐米纳米粒靶向治疗多发性骨髓瘤
pH-responsive bortezomib-loaded nanoparticles for targeted therapy of multiple myeloma
摘要
Abstract
Objective The BTZ@PLGA-RGD nanoparticles that target integrin αvβ3 and are loaded with bortezomib(BTZ)were prepared to achieve targeted therapy for multiple myeloma(MM).Methods The emul-sion solvent evaporation method and carbodiimide chemistry method were used to form BTZ@PLGA-RGD nanopar-ticles.The characterization of BTZ@PLGA-RGD nanoparticles was confirmed with scanning electron microscopy(SEM),dynamic light scattering(DLS),and fourier-transform infrared(FTIR).BTZ@PLGA-RGD nanoparticles were evaluated in terms of stability,encapsulation efficiency,release profile,and blood compatibility.The local-ization and cytotoxicity of BTZ@PLGA-RGD were determined using confocal laser scanning microscopy(CLSM)and the CCK-8 experiment.Results BTZ@PLGA-RGD nanoparticles were in a spherical shape with a uniform size distribution.The mean particle size of BTZ@PLGA-RGD nanoparticles was(113.7±3.1)nm,accompanied by a positive surface charge of(+6.4±1.3)mV.The entrapment efficiency of the BTZ@PLGA-RGD nanoparticles was 73.32％.These nanoparticles exhibited good long-term stability and blood compatibility.BTZ@PLGA-RGD nanoparticles had pH-responsive drug release properties,and the cumulative drug release(％)over 48 h was(82.34±0.12)％.FTIR analysis indicated that BTZ was successfully loaded into the BTZ@PLGA-RGD nanopar-ticles,and RGD was successfully immobilized on the surface of the nanoparticles.The localization analysis showed that the active targeting effect mediated by the RGD peptide enabled the BTZ@PLGA-RGD nanoparticles to be taken up by RPMI8226 cells more efficiently,and these nanoparticles could achieve lysosome escape.The CCK-8 experimental results indicated that BTZ@PLGA-RGD nanoparticles exhibited stronger cytotoxicity against RPMI8226 cells.Conclusion BTZ@PLGA-RGD demonstrates outstanding stability,water solubility,targeting ability,controlled drug-release properties,and biological safety,offering a novel research strategy for MM targeted therapy.关键词
硼替佐米/多发性骨髓瘤/纳米粒/表征/靶向治疗Key words
bortezomib/multiple myeloma/nanoparticles/characterization/targeting therapy分类
医药卫生引用本文复制引用
尹茉莉,罗文彬,罗思宇,林夕塞,陈三强,孙美艳..具有pH响应性载硼替佐米纳米粒靶向治疗多发性骨髓瘤[J].实用医学杂志,2026,42(6):959-968,10.基金项目
国家自然科学基金资助项目(编号:32573352) （编号:32573352）
吉林省科技厅项目(编号:20230101263JC) （编号:20230101263JC）
吉林省大学生创新创业训练项目(编号:S202413706028) （编号:S202413706028）
