时珍国医国药2026，Vol.37Issue(5)：807-812,6.DOI:10.70976/j.1008-0805.SZGYGY-2026-0502
滋肾祛瘀解毒方调控Nrf2/HO-1信号通路减轻子宫内膜异位症大鼠氧化应激的机制研究
A mechanistic study of Zishen Quyu Jiedu Formula(滋肾祛瘀解毒方)in alleviating oxidative stress in endometriosis rats via the Nrf2/HO-1 signaling pathway
摘要
Abstract
Objective To investigate the effect of Zishen Quyu Jiedu Formula(滋肾祛瘀解毒方,ZQJF)on endometriosis(EMs)by regulating the nuclear factor erythroid 2-related factor 2/heme oxygenase-1(Nrf2/HO-1)signaling pathway and to elucidate the thera-peutic mechanism from the perspective of oxidative stress.Methods Forty healthy non-pregnant female Sprague-Dawley(SD)rats were randomly divided into four groups(n=10 per group):sham-operated control,model(EMs),mifepristone,and ZQJF treatment.An EMs rat model was established via autologous endometrial transplantation.Hematoxylin-eosin(HE)staining was used to observe mor-phological changes in ectopic lesions.Immunohistochemistry(IHC)was performed to assess the positive expression of Nrf2,HO-1,and NAD(P)H quinone dehydrogenase 1(NQO1).Serum levels of superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px),8-epi-prostaglandin F2α(8-epi-PGF2α),interleukin-6(IL-6),tumor necrosis factor-alpha(TNF-α),and estradiol(E2)were measured by enzyme-linked immunosorbent assay(ELISA).Western blot(WB)was conducted to determine the protein expression levels of Nrf2,HO-1,NQO1,and Kelch-like ECH-associated protein 1(KEAP1)in endometrial tissues.Results Compared with the model group,the ZQJF and mifepristone groups showed significantly increased serum levels of SOD and GSH-Px(P<0.01),while levels of MDA,8-epi-PGF2α,and E2 were decreased(P<0.05).The volume of ectopic lesions was significantly reduced.Stromal cells in the ectopic tissue were more orderly arranged with attenuated inflammatory cell infiltration.The positive expression areas for Nrf2 and NQO1 were enlarged,and the protein expression levels of Nrf2,HO-1,and NQO1 were markedly upregu-lated(P<0.01).Conclusion ZQJF can alleviate endometriosis progression by mediating the Nrf2/HO-1 signaling pathway,which reduces inflammatory cell infiltration and promotes atrophy in ectopic endometrial tissues.This effect is associated with enhanced anti-oxidant expression of Nrf2,NQO1,and HO-1.These findings provide a scientific basis for further research on EMs.关键词
氧化应激/子宫内膜异位症/血瘀蕴毒/滋肾祛瘀解毒方/NRF2-HO1信号通路Key words
Oxidative stress/Endometriosis/Blood stasis and toxin accumulation/Zishen Quyu Jiedu Formula(滋肾祛瘀解毒方)/Nrf2/HO-1 signaling pathway分类
医药卫生引用本文复制引用
王若琳,田心阳,相珊,连方..滋肾祛瘀解毒方调控Nrf2/HO-1信号通路减轻子宫内膜异位症大鼠氧化应激的机制研究[J].时珍国医国药,2026,37(5):807-812,6.基金项目
国家自然科学基金面上项目(82174429) （82174429）
