时珍国医国药2026，Vol.37Issue(5)：813-820,8.DOI:10.70976/j.1008-0805.SZGYGY-2026-0503
二精丸改善糖尿病视网膜神经变性的机制研究
Mechanism of Erjing Pill(二精丸)in ameliorating diabetic retinal neurodegeneration
摘要
Abstract
Objective To investigate the mechanism by which Erjing Pill(二精丸,EJP)ameliorates diabetic retinal neurodegenera-tion(DRN)by activating moderate autophagy in Müller cells and regulating the AGEs/RAGE-Nrf2/HO-1 signaling pathway.Methods Streptozotocin-induced diabetic rat models were established and randomly divided into control group(CON),model group(MOD),cal-cium dobesilate group(CDC),and low/high-dose EJP groups(PL-L/H).After 12 weeks of intervention,ELISA was used to detect serum oxidative stress indices(SOD,MDA,GSH,CAT),GLU,retinal inflammatory cytokines(TNF-α,IL-6,IL-1β),and the con-tents of thioredoxin-interacting protein(TXNIP)and microtubule-associated protein LC3-Ⅱ in vitreous humor.Immunofluorescence(IF)was performed to detect the expression of caspase-3 and deposition of AGEs in retinal tissues.Western blot(WB)was used to mea-sure the protein levels of TXNIP,LC3-Ⅱ,Caspase-3,RAGE,and NF-κB in retinal tissues.RT-qPCR was employed to analyze the relative mRNA expression of RAGE,Nrf2,and HO-1 in retinal tissues.Key molecules involved in apoptosis,antioxidation,and autophagy regulation were analyzed.Results Following EJP intervention,the PL-H group exhibited significant upregulation of SOD,CAT,and GSH levels,alongside reduced levels of MDA,GLU,TNF-α,IL-6,IL-1β,RAGE,NF-κB,TXNIP,caspase-3,and LC3-Ⅱ.IF staining showed decreased fluorescence intensity of AGEs and caspase-3.TXNIP and LC3-Ⅱ levels in vitreous humor were also reduced.The relative mRNA expression of Nrf2 and HO-1 in retinal tissues showed an upward trend,while that of RAGE showed a downward trend.Conclusion EJP ameliorates DRN through a dual mechanism:regulating autophagic flux in Müller cells to alleviate glu-tamate excitotoxicity;inhibiting the AGEs/RAGE-mediated NF-κB inflammatory cascade and activating the Nrf2/HO-1 antioxidant defense system.关键词
糖尿病视网膜病变/谷氨酸/Müller细胞自噬/AGEs/RAGE-Nrf2/HO-1-信号通路Key words
Diabetic retinopathy/Glutamate excitotoxicity/Müller cell autophagy/AGEs/RAGE-Nrf2/HO-1 signaling path-way分类
医药卫生引用本文复制引用
王美霞,左祥铎,刘晓兰,徐翔,朱亚宁,李学芳,李静平..二精丸改善糖尿病视网膜神经变性的机制研究[J].时珍国医国药,2026,37(5):813-820,8.基金项目
国家自然科学基金委员会地区科学基金项目(82260795) （82260795）
云南省中医药基础研究联合专项重点项目(202301AZ070001-001) （202301AZ070001-001）
云南省中青年学术和技术带头人后备人才(202405AC350030) （202405AC350030）
云南省2021重大专项(202102AA310045) （202102AA310045）
云南中医药大学"培土生金"培育学科 （）
