时珍国医国药2026，Vol.37Issue(6)：1017-1024,8.DOI:10.70976/j.1008-0805.SZGYGY-2026-0603
清肺解毒化痰方抑制PINK1/Parkin通路介导的线粒体自噬治疗重症肺炎大鼠的机制研究
Mechanism of Qingfei Jiedu Huatan Formula(清肺解毒化痰方)in treating severe pneumonia rats via inhibiting PINK1/Parkin-mediated mitophagy
摘要
Abstract
Objective To elucidate the mechanism by which Qingfei Jiedu Huatan Formula(清肺解毒化痰方,QJHF)treats severe pneumonia in rats through inhibition of PINK1/Parkin-mediated mitophagy.Methods SD rats were randomly divided into four groups(n=20 each):control,model,QJHF,and Moxifloxacin(MOX).A severe pneumonia model was established using Klebsiella pneu-moniae.Lung tissues were collected on days 4 and 7 post-treatment for analysis.Hematoxylin-eosin(HE)staining was used to evaluate pathological changes,immunohistochemistry(IHC)to detect IL-6 and MCP-1 expressions,immunofluorescence to assess mitophagy levels(via LC3B and HSP60 co-localization),and RT-qPCR/Western blot(WB)to measure PINK1,Parkin,and VDAC1 mRNA/pro-tein expression.Results Compared with the control group,the model group exhibited alveolar wall rupture and fusion,inflammatory cell infiltration,significantly elevated lung injury scores(LIS)(P<0.01),upregulated IL-6 and MCP-1 expression(P<0.01),enhanced LC3B fluorescence,increased HSP60-LC3B co-localization(P<0.01),and elevated PINK1,Parkin,and VDAC1 mRNA/protein levels(P<0.01)on days 4 and 7.Compared with the model group,both QJHF and MOX groups showed reduced inflammatory cell infil-tration,decreased LIS(P<0.01),downregulated IL-6 and MCP-1(P<0.01),weakened LC3B fluorescence,reduced HSP60-LC3B co-localization(P<0.01),and suppressed PINK1,Parkin,and VDAC1 expression(P<0.01 or P<0.05).Therapeutic effects were time-dependent(P<0.01).Conclusion QJHF alleviates pulmonary pathological damage and preserves the lung function in severe pneu-monia rats by inhibiting excessive mitophagy via modulating the PINK1/Parkin pathway.关键词
重症肺炎/清肺解毒化痰方/线粒体自噬/PINK1/Parkin通路Key words
Severe pneumonia/Qingfei Jiedu Huatan Formula(清肺解毒化痰方)/Mitophagy/PINK1/Parkin pathway分类
医药卫生引用本文复制引用
井芃祯,贾茗棪,梁瀛今,张康,姬文帅,李亚,王海峰..清肺解毒化痰方抑制PINK1/Parkin通路介导的线粒体自噬治疗重症肺炎大鼠的机制研究[J].时珍国医国药,2026,37(6):1017-1024,8.基金项目
国家自然科学基金(82074411 （）
82274495) （）
国家中医药管理局高水平中医药重点学科(中医疫病学)(国中医药人教函[2023]85号) （中医疫病学）
河南省中医药科学研究专项课题(20-21ZYZD04 （）
2023ZY1005) （）
河南省科技研发计划联合基金(应用攻关类)项目(232301420084) （应用攻关类）
