中国草食动物科学2026，Vol.46Issue(2)：1-9,9.DOI:10.3969/j.issn.2095-3887.2025.00.073
牛胚胎发育相关子宫液外泌体miRNA的分析和筛选
Analysis and Screening of miRNAs in Uterine Fluid Exosomes Related to Bovine Embryo Development
摘要
Abstract
By analyzing the differences of miRNA expression in uterine fluid exosomes after superovulation in cow,this study aimed to explore their roles in early pregnancy and screen for miRNAs related to bovine embryo develop-ment and implantation.The research was conducted on Wandong young female cows,involving synchronized estrus,superovulation,and artificial insemination.On the seventh day after artificial insemination,the uterus was flushed to obtain embryos and flushing fluid.Three cows with more than 5.5 available embryos were selected as the experimental group,and three cows with no available embryos were chosen as the control group,based on the international average level of 5.5 embryos.Exosomes were extracted from the uterine flushing fluid using ultracentrifugation and identified by transmission electron microscopy.miRNAs in the exosomes were extracted and their expression levels were de-tected,and miRNAs with significant differences were analyzed.Through target gene prediction and analysis,miRNAs related to embryo development and implantation were screened and identified.The transmission electron microscopy results showed that the detected samples were cup-shaped,consistent with the characteristics of exosomes.Compared with the control group,five miRNAs were significantly upregulated and ten were significantly downregulated in the ex-perimental group(P<0.05).The most significantly different known miRNA was bta-miR-1(P=0.001).The 15 differen-tially expressed miRNAs were predicted 2 903 non-repetitive target genes.KEGG analysis results indicated that these target genes were mainly enriched in the MAPK,Rap1 and focal adhesion pathways,suggesting that uterine fluid exo-some miRNAs may regulate embryo development and implantation.Overexpression of bta-miR-1 inhibited the mRNA and protein levels of the IGF1 gene,indicating a possible targeting relationship between bta-miR-1 and the IGF1 gene.The research results can provide a reference for screening uterine fluid exosome miRNAs related to embryo development and implantation and lay a foundation for understanding their regulatory mechanisms in early pregnancy.关键词
牛/外泌体/miRNA/差异表达/妊娠Key words
cattle/exosome/miRNA/differential expression/pregnancy分类
农业科技引用本文复制引用
栾曼茹,吕世杰,朱肖亭,闵佳,张子敬,施巧婷,鲁立刚,刘洪瑜,闫祥洲,王二耀..牛胚胎发育相关子宫液外泌体miRNA的分析和筛选[J].中国草食动物科学,2026,46(2):1-9,9.基金项目
国家肉牛牦牛产业技术体系(CARS-37) （CARS-37）
河南省肉牛产业技术体系项目(HARS-22-13-S) （HARS-22-13-S）
河南省科技攻关(252102110073) （252102110073）
河南省自然基金项目(252300420687) （252300420687）
河南省农业科学院自主创新项目(2025ZC66) （2025ZC66）
河南省农业科学院自主创新项目(2025ZC67) （2025ZC67）
河南省农业科学院科技创新团队项目(2024TD25) （2024TD25）
河南省牛产业科技特派员服务团(泌阳) （泌阳）
神农种业实验室协同创新项目(SN02-2025-02) （SN02-2025-02）
