中国草食动物科学2026，Vol.46Issue(2)：10-20,11.DOI:10.3969/j.issn.2095-3887.2025.00.053
高原型藏羊皮肤组织转录组分析揭示毛囊发育关键调控基因
Transcriptome Analysis of Skin Tissue in Plateau-type Tibetan Sheep Reveals Key Regulatory Genes for Hair Follicle Development
摘要
Abstract
This study aims to identify key candidate genes regulating hair follicle development through transcriptome sequencing analysis of skin tissues associated with different wool fiber characteristics,thereby provding a crucial theo-retical basis for an in-depth elucidation of molecular mechanisms underlying wool trait development in Tibetan sheep.Skin tissues from adult plateau-type Tibetan sheep and Oula sheep were selected for paraffin sectioning and HE stain-ing.High-throughput sequencing(PE150)was conducted using the Illumina NovaSeq platform.Differential expression genes(DEGs)were analyzed using DESeq2(|log2FC|>1,FDR<0.05),and functional enrichment analysis was per-formed in combination with the GO and KEGG databases.The expression of candidate genes was verified by qRT-PCR.Morphological studies on hair follicle indicated that primary hair follicles possess a larger diameter and a com-plete structure,while secondary hair follicles are characterized by a smaller diameter and a higher quantity.Addition-ally,the hair follicle density of plateau-type Tibetan sheep was relatively low,with a larger distance between hair fol-licles;in contrast,Oula sheep exhibit a higher hair follicle density,with a smaller distance between hair follicles.Tran-scriptome sequencing results showed that a total of 1 065 DEGs were identified,including 434 up-regulated genes and 631 down-regulated genes.Through GO and KEGG functional enrichment analysis,it was found that these DEGs were significantly enriched in the Wnt signaling pathway,extracellular matrix receptor interaction,PPAR signaling pathway,adhesion,and PI3K-Akt signaling pathway.Among them,key genes such as THBS3,COL6A3,COL6A2,COL6A1,COL1A1,COL1A2,OVOL2,MYOC,FABP3,MMP1,KIT,FGF5,DKK1,MMP7,WNT7B and WNT16 played important roles in the regulation of hair follicle development.The qRT-PCR results showed that the Pearson correlation coeffi-cient of the selected 7 DEGs was 0.77,and the P value was 3.95×10-5,indicating that the RNA-seq data had high reli-ability.The identified key genes including THBS3,COL6A3,COL6A2,COL6A,WNT7B and WNT16 exhibited significant differential expression patterns,providing an important theoretical basis for revealing the molecular mechanism of hair follicle development in Tibetan sheep.关键词
高原型藏羊/皮肤组织/形态学/转录组Key words
plateau type Tibetan sheep/skin tissues/morphology/transcriptome分类
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裴全帮,左明星,李蓉,徐腾,祁军英,田得红..高原型藏羊皮肤组织转录组分析揭示毛囊发育关键调控基因[J].中国草食动物科学,2026,46(2):10-20,11.基金项目
青海省自然科学基金项目(2023-ZJ-748) （2023-ZJ-748）
