中国草食动物科学2026，Vol.46Issue(2)：21-29,9.DOI:10.3969/j.issn.2095-3887.2025.00.067
帕米尔牦牛FGF10基因克隆及组织表达
Cloning and Tissue Expression of FGF10 Gene in Pamir Yak
摘要
Abstract
This study aimed to investigate the structure and function of the yak FGF10 gene and explore its expression patterns in eight tissues of Pamir yak.This experiment used the cDNA of the muscle tissue of Pamir yak as the template and cloned the CDS region of the FGF10 gene by PCR technology.The gene sequence was analyzed by bioinformatics methods,and the expression level of the FGF10 gene in eight different tissue samples of Pamir yak was quantitatively detected by qRT-PCR.The results indicated that the coding sequence(CDS)region of the FGF10 gene in Pamir yak was 642 bp in length and encoded a total of 213 amino acids.The homology comparison results showed that the simila-rity between Pamir yak and wild yak as well as domestic cattle is up to 100%;And the lowest similarity is the house mouse,which was 90.1%.The results of bioinformatics analysis showed that the molecular formula of the FGF10 pro-tein in Pamir yak was C1049H1639N299O307S13,and the total number of atoms,instability index and total average hydro-philic index were 3 307,45.07 and-0.358,respectively,indicating that this protein was a stable hydrophilic protein.Signal peptide analysis confirmed that FGF10 protein is a secreted protein containing transmembrane helical structures.Subcellular localization analysis revealed that this protein is primarily distributed in the extracellular environment.Its molecular structure included two N-glycosylation sites and 47 potential phosphorylation sites.Furthermore,the secon-dary structure of the FGF10 protein is predominantly composed of random coils,and it interacts with proteins such as FGFR1,FGFR2,and FLT1.Analysis of the expression patterns of the FGF10 gene in 8 tissues indicated differences among the tissues,among which the relative expression level in longissimus dorsi tissue was the highest,and signifi-cantly higher than the other 7 tissues(P<0.01).关键词
帕米尔牦牛/FGF10基因/基因克隆/组织表达Key words
Pamir yak/FGF10 gene/gene cloning/tissue expression分类
农业科技引用本文复制引用
邓婧瑛,贾建磊,陈倩,王佟,于沁冉,张明昊,马超凡,李宁,包鹏甲,阎萍..帕米尔牦牛FGF10基因克隆及组织表达[J].中国草食动物科学,2026,46(2):21-29,9.基金项目
青海省昆仑英才高端创新创业人才培养拔尖人才项目(QHKLYC-GDCXCY-2023-154) （QHKLYC-GDCXCY-2023-154）
青海大学实验(实践)课程建设项目(SYKC-2023-03) （实践）
深圳市科技计划项目(KCXF20201221173205015) （KCXF20201221173205015）
新疆天池英才项目(CTYC-TP2023) （CTYC-TP2023）
