中国草食动物科学2026，Vol.46Issue(2)：35-46,12.DOI:10.3969/j.issn.2095-3887.2025.00.078
不同苜蓿品种萌发期和苗期耐盐性评价
Evaluation of Salt Tolerance of Different Alfalfa Varieties at Germination and Seedling Stages
摘要
Abstract
To investigate the effects of NaCl treatment on the growth and physiological characteristics of different al-falfa varieties during germination and seedling stages,this study utilized five varieties(Banongke No.1,Aohan,Zhongmu No.3,Zhongtian No.3,and Zangmu No.1)as experimental materials.During the germination stage,treat-ments included 0%(control),0.4%,0.8%,and 1.2%NaCl,with growth indices measured accordingly.At the seedling stage,growth performance under 0%(control),0.4%,and 0.8%NaCl treatments was evaluated,and changes in antioxi-dant enzyme activities,osmoregulatory substances,and Na⁺/K⁺accumulation were analyzed.The results indicated that during germination stage,as NaCl concentration increased,germination rate,germination potential,vigor index,root length,shoot length,and fresh seedling weight of all five varieties declined.The 0.8%NaCl treatment significantly in-hibited seed germination and growth across all cultivars,with Banongke No.1 exhibiting the highest germination poten-tial,showing a statistically significant difference(P<0.05)compared to the other four varieties.The 1.2%NaCl treat-ment severely suppressed germination,reducing the germination rate of Zhongtian No.3 to nearly zero.At the seedling stage,increasing NaCl concentrations inhibited the growth of all alfalfa varieties.However,Banongke No.1 showed a significant increase in root biomass compared to control under elevated NaCl levels,with a marked difference(P<0.05)from the other four varieties at 0.8%NaCl treatments.Na⁺content in leaves,stems,and roots generally increased with the increase of NaCl concentration for all alfalfa,showing significant differences from control(P<0.05).Under 0.8%NaCl treatment,Banongke No.1 displayed the smallest increments in Na⁺content in leaves and stems compared to con-trol,with values of 30.84 mmol/g and 40.5 mmol/g,respectively.With rising NaCl concentrations,K⁺content in stems and roots,as well as the K⁺/Na⁺ratio in stems,roots and leaves of all alfalfa generally decreased.With rising NaCl con-centrations,malondialdehyde content initially increased and then declined,soluble protein content decreased progres-sively,while proline content,superoxide dismutase,peroxidase,and catalase activities all increased,showing signifi-cant differences from control(P<0.05)under 0.8%NaCl treatment.Based on a comprehensive salt tolerance evalua-tion,the ranking of salt tolerance during germination stage was Banongke No.1>Zhongmu No.3>Zangmu No.1>Aohan>Zhongtian No.3;and at the seedling stage,the order was Banongke No.1>Zhongmu No.3>Zhongtian No.3>Zangmu No.1>Aohan.These results indicate that Banongke No.1 exhibits stronger salt tolerance than Zhongmu No.3,Zangmu No.1 surpasses Aohan in salt tolerance,and Zhongtian No.3 demonstrates greater sensitivity to NaCl during germina-tion stage than at the seedling stage.关键词
苜蓿/萌发期/苗期/耐盐性Key words
alfalfa/germination stage/seedling stage/salt tolerance分类
农业科技引用本文复制引用
李玉杰,杨红善,张瑞欣,吴芳,刘琳,崔光欣,王杨,段慧荣..不同苜蓿品种萌发期和苗期耐盐性评价[J].中国草食动物科学,2026,46(2):35-46,12.基金项目
农业生物育种重大专项(2022ZD04011) （2022ZD04011）
内蒙古自治区"揭榜挂帅"项目专题(2022JBGS00160104) （2022JBGS00160104）
甘肃省科技计划项目-甘肃重要乡土草及牧草种质创新与品种选育(23ZDKA013) （23ZDKA013）
自治区科技支疆项目计划(2024E02024) （2024E02024）
中国农业科学院兰州畜牧与兽药研究所创新工程专项资金项目(CAAS-LMY-04) （CAAS-LMY-04）
兰州市青年科技人才创新项目(2024-QN-4) （2024-QN-4）
