中国草食动物科学2026，Vol.46Issue(2)：58-66,9.DOI:10.3969/j.issn.2095-3887.2025.00.045
马红球菌重组ABC转运蛋白的表达及其免疫效果评价
Expression of Recombinant ABC Transporter Protein from Rhodococcus equi and Evaluation of Its Immunological Effect
摘要
Abstract
Rhodococcus equi(R.equi)is an intracellular parasitic bacterium that causes septic bronchopneumonia in foals,resulting in significant economic losses to the equine industry.Virulence-associated protein A(VapA)is a key virulence factor for R.equi,although studies have demonstrated that immunisation of animals with VapA has a pro-tective effect against R.equi infections,a commercial vaccine against R.equi has not yet been investigated.Bioinfor-matics analyses showed that the ABC transporter protein of R.equi could be a potential protective antigen against R.equi infection.In this study,recombinant ABC transporter protein(rABC)was prepared by prokaryotic expression,and BALB/c mice were immunised with rABC,recombinant VapA(rVapA,positive control)and PBS(negative con-trol)emulsified with Freund's adjuvant,respectively,to test their immunisation effects.The results showed that rABC with the expected molecular weight(62 kDa)and purified was obtained;specific antibodies began to be pro-duced 14 d after immunisation with rABC and rVapA;serum IgG antibody P/N values were≥15.0 at 42 d post-immunisation,IgG1 and IgG2a antibody potencies were 1∶409 600 and 1∶102 400,respectively,and the proportion of TNF-α-positive CD4+and CD8+T-cell in the spleen were significantly higher than those of the negative control group(P<0.05);7 d after infecting the bacteria,TNF-α and IL-6 secretion of splenocytes were significantly higher in rABC immunised group compared with those of the negative control group(P<0.05),and the histopathological changes in the lungs were attenuated.The results of this study suggest that ABC transporter proteins induce strong humoral and cellu-lar immune responses and attenuate lung pathological damage in mice,and are potential candidate antigens for R.equi subunit vaccine development.关键词
马红球菌/ABC转运蛋白/亚单位疫苗/免疫效果Key words
Rhodococcus equi/ABC transporter protein/subunit vaccine/immunological efficacy分类
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高仕文,赵红琼,李国庆,汪香玉,顾伟芳,唐健,郭丁诺雅,仙宗萍,古丽米热·阿不力米提,刘璐..马红球菌重组ABC转运蛋白的表达及其免疫效果评价[J].中国草食动物科学,2026,46(2):58-66,9.基金项目
新疆维吾尔自治区区域协同创新专项上海合作组织科技伙伴计划及国际科技合作计划(2022E01027) （2022E01027）
