中国草食动物科学2026，Vol.46Issue(2)：67-74,8.DOI:10.3969/j.issn.2095-3887.2025.00.058
甘肃张掖、金昌地区犊牛腹泻致病菌的分离鉴定、致病性及耐药性分析
Isolation,Identification,Pathogenicity and Drug Resistance Analysis of Pathogenic Bacteria from Diarrhea Calves in Zhangye and Jinchang,Gansu Province
摘要
Abstract
This study aimed to investigate the species,pathogenicity,and antibiotic resistance prevalence of the primary pathogenic bacteria from diarrhea calves in Zhangye and Jinchang regions of Gansu province,providing a reference for local prevention and treatment.A total of 41 samples(27 fecal samples,4 milk samples,10 soil samples)were collected from 6 cattle farms in Zhangye and Jinchang.Pathogenic bacteria were isolated and identified by bacterial culture,Gram staining,growth characteristic identification,and 16S rRNA gene sequencing.Pathogenicity tests and Kirby-Bauer(K-B)disk diffusion were utilized to confirm pathogenicity and antibiotic resistance.The results showed that 4 pathogenic strains were isolated from 41 samples,among which JC01,JC02,and JC03 were identified as Escherichia coli,and JC04 was identified as Klebsiella pneumoniae.Homology analysis indicated that JC01 was most closely re-lated to Escherichia coli strain U 5/41,while JC04 showed the highest homology with the standard strain Klebsiella pneumoniae subsp.ozaenae.Pathogenicity studies indicated that both JC01 and JC04 exhibited strong pathogenicity,causing severe pathological damage to the spleen and jejunum.Antibiotic susceptibility testing revealed that the 3 E.coli strains exhibited high resistance to β-lactams(88.89%),macrolides(100%),tetracyclines(100%),lincosamides(100%),and sulfonamides(100%).The K.pneumoniae strain was fully resistant to β-lactams,aminoglycosides,macro-lides,tetracyclines,lincosamides,and sulfonamides,remaining susceptible only to cefoxitin,ofloxacin,and norfloxa-cin.In conclusion,the primary pathogens causing diarrhea in calves in the Zhangye and Jinchang were E.coli and K.pneumoniae,both of which exhibited strong pathogenicity and broad-spectrum,high-level antibiotic resistance.关键词
犊牛腹泻/大肠杆菌/肺炎克雷伯菌/分离鉴定/耐药性Key words
calf diarrhea/Escherichia coli/Klebsiella pneumoniae/isolation and identification/antibiotic resistance分类
农业科技引用本文复制引用
顾强文,陈茜,吴延荣,高强,徐进鹏,文洋,王胜义,郝宝成..甘肃张掖、金昌地区犊牛腹泻致病菌的分离鉴定、致病性及耐药性分析[J].中国草食动物科学,2026,46(2):67-74,8.基金项目
甘肃省青年人才(个人项目)(2025QNGR59) （个人项目）
中国农业科学院科技创新工程兽用天然药物与抗生素替代团队项目(25-LZIHPS-03) （25-LZIHPS-03）
