中国生物防治学报2026，Vol.42Issue(1)：10-22,13.DOI:10.16409/j.cnki.2095-039x.2025.02.068
复合菌群对黄芪根腐病的防效及对根际微生物群落的影响
Control Effect of Composite Microbial Consortium on Astragalus membranaceus Root Rot and Its Influence on Microorganism Community in Rhizosphere Soil
摘要
Abstract
To screen composite microbial consortium for the control of Astragalus membranaceus root rot,antagonistic strains were screened from the rhizosphere soil of A.membranaceus using the gradient dilution and plate confrontation methods.The strains were further identified based on morphological,physiological and biochemical characteristics and molecular biology methods.The antagonistic strains were combined to construct a consortium,and its effects on defense related enzymes in A.membranaceus roots were evaluated.The influence of the composite microbial consortium on soil bacterial community was analyzed using high-throughput sequencing technology.The results showed that strains 6-35,17-27 and 62-313 could significantly inhibit Fusarium oxysporum,with the inhibition zone width of 6.5,8.4 and 4.2 mm,respectively.Strains 6-35,17-27 and 62-313 were identified as Bacillus amyloliquefaciens,Paenibacillus polymyxa and B.cereus,respectively.The optimal fermentation ratio was 3∶1∶1,with an inhibition rate of 40.90％.The composite microbial consortium showed the control efficacy of 72.17％and 80.36％at 7 and 14 d after inoculation on A.membranaceus root rot,respectively.After treatment with the composite microbial consortium,the activities of chitinase,peroxidase(POD),superoxide dismutase(SOD),phenylalanine ammonialyase(PAL),andcatalase(CAT)significantly increased in A.membranaceus roots.The application of composite microbial consortium could increase α and β diversity of rhizosphere bacterial communities,and various beneficial microorganisms such as Gemmatimonas and Streptomyces in the treatment soil was significantly higher than that in the control soil.The composite microbial consortium had excellent control effects on A.membranaceus root rot,and might be used for the development and application of biological control reagent.关键词
拮抗细菌/鉴定/复合菌群/防御酶/微生物多样性Key words
antagonistic bacterium/identification/composite microbial consortium/defense enzyme/microbial diversity分类
农业科技引用本文复制引用
王燕,赵蕊,郜雨晴,李江波,王春伟..复合菌群对黄芪根腐病的防效及对根际微生物群落的影响[J].中国生物防治学报,2026,42(1):10-22,13.基金项目
山西省现代农业产业技术体系建设专项(2025CYJSTX16-ZYC22) （2025CYJSTX16-ZYC22）
