中国生物防治学报2026，Vol.42Issue(1)：172-179,8.DOI:10.16409/j.cnki.2095-039x.2025.07.005
基于发泡聚丙烯载体的爪哇虫草MSC-F1固态发酵工艺优化
Optimization of Solid-state Fermentation Process for Cordyceps javanica MSC-F1 Based on Expanded Polypropylene Carrier
摘要
Abstract
Cordyceps javanica MSC-F1 is a biocontrol fungus with significant pathogenicity against various pests such as Diaphorina citri and Panonychus citri.However,its industrialized application is hindered by high fermentation cost and imperfect fermentation processes.To enhance spore yield and reduce production cost,this study employed expanded polypropylene particles(EPP)as the primary inert carrier and optimized medium composition and culture conditions using single-factor and orthogonal test for high spore yield.The results indicated that the optimal basal medium consisted of a mixture of EPP and rice husk at a ratio of 4∶1(V/V),with a solid-to-liquid ratio of 1∶1.2.Additionally,the basal medium was supplemented with 20％corn flour,16％soybean meal,and 1.5％yeast extract.The optimal solid-state fermentation conditions were:cultivation temperature at 25 ℃,fermentation period for 9 days,inoculation size of 2％,and initial pH of 7.0.Under these optimized conditions,the spore yield increased 6.28 times compared to pre-optimization level.This study provides critical theoretical support for solid-state fermentation of Cordyceps javanica using EPP as inert carrier.关键词
爪哇虫草/固态发酵/发泡聚丙烯/惰性载体Key words
Cordyceps javanica/solid-state fermentation/expanded polypropylene/inert carrier分类
农业科技引用本文复制引用
杜丹超,蒲占湑,刘顺民,朱莉,胡秀荣,吕佳,鹿连明..基于发泡聚丙烯载体的爪哇虫草MSC-F1固态发酵工艺优化[J].中国生物防治学报,2026,42(1):172-179,8.基金项目
浙江省科技计划(2023C04030,2023C02057) （2023C04030,2023C02057）
国家现代农业(柑橘)产业技术体系(CARS-26) （柑橘）
